Sterilization by pure oxygen plasma and by oxygen-hydrogen peroxide plasma: an efficacy study

International Journal of Pharmaceutics
M R BoscariolT J A Pinto

Abstract

Plasma is an innovative sterilization method characterized by a low toxicity to operators and patients, and also by its operation at temperatures close to room temperatures. The use of different parameters for this method of sterilization and the corresponding results were analyzed in this study. A low-pressure inductive discharge was used to study the plasma sterilization processes. Oxygen and a mixture of oxygen and hydrogen peroxide were used as plasma source gases. The efficacy of the processes using different combinations of parameters such as plasma-generation method, type of gas, pressure, gas flow rate, temperature, power, and exposure time was evaluated. Two phases were developed for the processes, one using pure oxygen and the other a mixture of gases. Bacillus subtilis var. niger ATCC 9372 (Bacillus atrophaeus) spores inoculated on glass coverslips were used as biological indicators to evaluate the efficacy of the processes. All cycles were carried out in triplicate for different sublethal exposure times to calculate the D value by the enumeration method. The pour-plate technique was used to quantify the spores. D values of between 8 and 3 min were obtained. Best results were achieved at high power levels (350 and 40...Continue Reading

References

Oct 6, 1997·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·M J AlfaN Olson

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Citations

Jul 22, 2011·Biofouling·Christian Traba, Jun F Liang
Sep 27, 2018·Frontiers in Microbiology·Gaëlle CarréMarie-Paule Gelle

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