Sterilizing Bacillus pumilus spores using supercritical carbon dioxide

Journal of Microbiological Methods
Jian ZhangYuehuei H An

Abstract

Supercritical carbon dioxide (SC CO(2)) has been evaluated as a new sterilization technology. Results are presented on killing of B. pumilus spores using SC CO(2) containing trace levels of additives. Complete killing was achieved with 200 part per million (ppm) hydrogen peroxide in SC CO(2) at 60 degrees C, 27.5 MPa. Addition of water to SC CO(2) resulted in greater than three-log killing, but this is insufficient to claim sterilization. Neither ethanol nor isopropanol when added to SC CO(2) affected killing.

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Citations

Sep 9, 2010·Journal of Industrial Microbiology & Biotechnology·Claudio Cinquemani
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