Steroid hormone receptors activate transcription in glial cells of intact retina but not in primary cultures of retinal glial cells

Journal of Molecular Neuroscience : MN
Y C LiA P Young

Abstract

We have compared the steroid responsiveness of Müller glial cells of intact embryonic chicken retina with that of primary cultures derived from Müller glia. Appropriately constructed fusion genes were found to be highly glucocorticoid inducible after their cotransfection with an expression vector encoding the human glucocorticoid receptor (GR) into intact embryonic d-10 (E10) or E5.5 retina. Dramatically attenuated inductions were obtained after contransfection of Müller cell primary cultures. The progesterone receptor (PR) was also demonstrated to function in intact retina, but not in Müller cell primary cultures. An immunochemical assay was utilized to confirm that a glucocorticoid-responsive, beta-galactosidase-encoding fusion gene was specifically induced in Müller cells after its transfection into intact retina. Thus, in contrast to Müller cells in intact retina, Müller cells in primary culture have lost the capacity to achieve transcriptional activation by steroid receptors. We postulate that coordinate expression of the GR, and other more general factors required for steroid inducibility, is lost by dispersion and primary culture of retinal Müller glial cells.

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Citations

Oct 19, 2002·Journal of Neuroscience Methods·Bernd BiedermannThomas Pannicke
Mar 20, 2010·Neurochemical Research·Florian NeumannAndreas Bringmann
Oct 9, 2015·Journal of Neurochemistry·Alice C Wyse JacksonThomas G Cotter
Aug 24, 1999·Journal of Neurobiology·L VardimonL Shiftan
Jan 23, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Peter KoulenMeharvan Singh
Dec 18, 2001·American Journal of Medical Genetics·M M Cohen

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