Steroid receptor coactivator-1 interacts with the p50 subunit and coactivates nuclear factor kappaB-mediated transactivations.

The Journal of Biological Chemistry
S Y NaJ W Lee

Abstract

Steroid receptor coactivator-1 (SRC-1) specifically bound to the transcription factor NFkappaB subunit p50 but not to p65 as demonstrated by the yeast two hybrid tests and glutathione S-transferase pull down assays. The p50-binding site was localized to a subregion of SRC-1 (amino acids 759-1141) that encompasses the previously described CBP-p300-binding domain. In mammalian cells, SRC-1 potentiated the NFkappaB-mediated transactivations in a dose-dependent manner. Coexpression of p300 further enhanced this SRC-1-potentiated level of transactivations, consistent with the recent findings in which CBP and p300 were shown to be transcription coactivators of the p65 subunit (Perkins, N. D., Felzien, L. K., Betts, J. C., Leung, K., Beach, D. H., and Nabel, G. J. (1997) Science 275, 523-527; Gerritsen, M. E., Williams, A. J., Neish, A. S. , Moore, S., Shi, Y., and Collins, T. (1997) Proc. Acad. Natl. Sci. U. S. A. 94, 2927-2932). These results suggest that at least two distinct coactivator molecules may cooperate to regulate the NFkappaB-dependent transactivations in vivo and SRC-1, originally identified as a coactivator for the nuclear receptors, may constitute a more widely used coactivation complex.

References

Nov 15, 1995·Genes & Development·I M VermaS Miyamoto
Dec 1, 1993·Trends in Genetics : TIG·T D Gilmore, P J Morin
Oct 28, 1993·Nature·J C ChriviaR H Goodman
Dec 15, 1995·Cell·D J MangelsdorfR M Evans
Jan 1, 1996·Annual Review of Immunology·A S Baldwin
Sep 5, 1996·Nature·D ChakravartiR M Evans
Sep 26, 1996·Nature·S BhattacharyaD M Livingston
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·T P YaoD M Livingston
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·B HansteinM Brown
Dec 19, 1996·Nature·A J Bannister, T Kouzarides
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J ZhangJ E Darnell
Apr 11, 1997·The Journal of Biological Chemistry·R WeilA Israël
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·M E GerritsenT Collins
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·H LiJ D Chen
Sep 20, 1997·Nature·T E SpencerB W O'Malley
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·K De BosscherG Haegeman

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Citations

Mar 10, 2000·Biochemical and Biophysical Research Communications·Y YanagiS Kato
Mar 11, 2011·Breast Cancer Research and Treatment·Melanie SpearsJohn M S Bartlett
Jun 8, 2002·Biochemical and Biophysical Research Communications·Jonathan H DennisDavid S Latchman
Jun 1, 2002·Molecular and Cellular Endocrinology·Isao KuriharaTakao Saruta
Mar 14, 2000·Gene·C Leo, J D Chen
May 7, 2003·The Journal of Steroid Biochemistry and Molecular Biology·C PunyadeeraP Groothuis
Sep 28, 2002·International Immunopharmacology·Xia Zhang, Howard A Young
Jan 5, 2002·Breast Cancer Research and Treatment·D O AzorsaP S Meltzer
Apr 7, 2005·The Journal of Biological Chemistry·Zhanguo GaoJianping Ye
Mar 22, 2001·EMBO Reports·D A Chamovitz, D Segal
Nov 29, 2002·The EMBO Journal·Lin-feng ChenWarner C Greene
Aug 21, 2007·Nucleic Acids Research·Yong Seok ChoiSunjoo Jeong
Dec 1, 2005·Kidney International·Xiong Z RuanJohn F Moorhead
Apr 25, 2002·Molecular and Cellular Biology·Ray-Chang WuBert W O'Malley
Aug 23, 2002·Molecular and Cellular Biology·Majdi S QutobJoseph Torchia
Jan 17, 2003·Molecular and Cellular Biology·Yolanda BayonF Javier Piedrafita
Dec 31, 2005·Molecular and Cellular Biology·Jamie E HobergMarty W Mayo
Aug 12, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Wei Xu
May 20, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dongyuan LiuCarole R Mendelson
May 5, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Toni R Bartling, Mitchell L Drumm
Mar 8, 2006·Reviews in the Neurosciences·Thierry D Charlier, Jacques Balthazart
Mar 16, 2012·International Journal of Biological Sciences·Claire A WalshJianming Xu
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·J AnD C Leitman
Apr 8, 2014·International Journal of Molecular Sciences·Kairit TintsKaia Palm
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·K De BosscherG Haegeman
Jul 25, 2006·Experimental Cell Research·Enkeleda NakuciWael M ElShamy
Feb 13, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Rika OuchidaHirotoshi Tanaka
Feb 11, 2009·The Biochemical Journal·Helena CvijicFriedemann Horn
Jun 19, 2014·Nucleic Acids Research·Alessandra Di LorenzoMark T Bedford

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