PMID: 9178804May 16, 1997Paper

Steroid receptor measurement in breast cancers: comparison between ligand binding and enzyme-immunoassay in cytosolic and nuclear extracts

International Journal of Cancer. Journal International Du Cancer
Y A LuqmaniA H Parkar

Abstract

We have analysed cytoplasmic and nuclear extracts of breast-cancer tissue from a total of 799 patients, measuring both oestrogen and progesterone receptors (ER, PR) using either the ligand binding assay (LBA) or the enzyme immunoassay technique (EIA). Mean and median receptor levels were much lower than those widely reported by others. For ER, this may in part be a consequence of the younger median age of the patient group. The frequency of positivity, using consensus cut-off values for clinical evaluation, was also lower than that reported by the EORTC Receptor Study Group. Although the measurements comparing the 2 methods were statistically correlated in terms of positivity, based on the above criteria for clinical assessment, concordance was considered to be relatively poor, particularly for ER when assayed in the same samples by the 2 methods. In cytosolic but not nuclear extracts, the LBA method gave a higher median value for ER than the EIA (except in the group that had EIA values greater than 15 fmol/mg protein); for PR, median values were higher with EIA in both cell fractions. There was an excellent correlation between receptor amounts in cytosolic and nuclear extracts for both ER and PR using the EIA; this was signifi...Continue Reading

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Citations

Feb 18, 2004·The Breast : Official Journal of the European Society of Mastology·L TemmimM Mathew
Aug 2, 2003·Breast Cancer Research and Treatment·Angelos D GritzapisMichael Papamichail
Oct 7, 1998·International Journal of Cancer. Journal International Du Cancer·L HeflerC Kainz

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