Steroid specificity of the glucocorticoid inhibition of amino acid transport in rat hepatoma cells

Endocrinology
T D Gelehrter, R A McDonald

Abstract

Dexamethasone, a synthetic glucocorticoid, inhibits the initial rate of transport of the nonmetabolizable amino acid, alpha-aminoisobutyric acid, in rat hepatoma tissue culture (HTC) cells. To determine whether this inhibition is mediated by the same proximal steps as is the steroidal induction of tyrosine aminotransferase, we have examined the hormonal specificity of these two responses for various steroids previously characterized with respect to transaminase induction as agonists, partial agonists, antagonists, or inactive steroids. We conclude that the steroidal inhibition of amino acid transport, at steroid concentrations of 10(-5) M or less is mediated by the same glucocorticoid receptor mechanisms as the induction of tyrosine aminotransferase. First, the concentrations at which the full agonists, dexamethasone and cortisol, produce their half-maximal effects on phenomena are the same. Second, tetrahydrocortisol, which does not interact with the glucocorticoid receptor, neither inhibits transport nor induces transaminase. Third, the competitive interactions between partial agonists or antagonists and the full agonist dexamethasone with respect to both transport inhibition and enzyme induction are virtually identical. At c...Continue Reading

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