Steroid therapy can modulate gut barrier function, host defense, and survival in thermally injured mice

The Journal of Surgical Research
L GianottiT Pyles

Abstract

Prednisone may be immunosuppressive and dehydroepiandrosterone may stimulate the immune response, but their effect on gut-origin sepsis caused by bacterial translocation has not been studied. Balb/c mice were treated orally with prednisone (1 or 10 mg/kg/day) or saline for 4 days before receiving gavage with 10 (10) 14 C-labeled Escherichia coli and a 20% thermal injury. Mice were transfused with allogeneic blood and given dehydroepiandrosterone (5 or 25 mg/kg/day) or vehicle subcutaneously for 4 days before bacterial gavage and thermal injury. Some groups in each experiment were observed 10 days for mortality and others were sacrificed 4 hr postburn to measure translocation and survival of translocated bacteria. Survival in prednisone treated animals was 25% (10 mg/kg/day) and 75% (1 mg/kg/day) versus 80% for controls. Following dehydroepiandrosterone administration, survival was 72% (25 mg/kg/day/group) and 30% (5 mg/kg/day/group) versus 16% for controls. High dose prednisone increased bacterial translocation to the intestinal wall and mesenteric lymph nodes and greatly impaired killing of translocated E. coli. In contrast, dehydroepiandrosterone (25 mg/kg) did not affect translocation but significantly improved bacterial kil...Continue Reading

Citations

Jul 15, 2009·Critical Care : the Official Journal of the Critical Care Forum·Tanja BarkhausenMartijn van Griensven
Feb 12, 2008·Experimental and Molecular Pathology·V K SinghM H Whitnall
Aug 31, 2010·Cell Biochemistry and Function·Metehan GümüşFatih Akçay
Dec 24, 2011·International Journal of Radiation Biology·Vijay K SinghMark H Whitnall
Jul 20, 2016·Biochemical Pharmacology·Borja OcónFermín Sánchez de Medina
May 11, 2005·The Journal of Surgical Research·Scott BantaMartin L Yarmush
Sep 3, 2004·Current Gastroenterology Reports·John Simpson
Nov 2, 2001·JPEN. Journal of Parenteral and Enteral Nutrition·S Ruiz-SantanaB N Díaz-Chico
Sep 30, 2003·The British Journal of Surgery·C R MorrisA R Hart
May 16, 2000·The American Journal of Gastroenterology·R CaprilliG Frieri
Jan 4, 2003·Immunopharmacology and Immunotoxicology·Mark H WhitnallWilliam E Jackson

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