PMID: 44773Jan 1, 1979

Steroid-transforming enzymes from microorganisms. XI. Reversibility of the dehydrogenation reaction of the steroid-1-dehydrogenase from Nocardia opaca

Zeitschrift für allgemeine Mikrobiologie
H GrohC Hörhold

Abstract

Highly purified preparations of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca have been investigated in both types of reactions: 1.2-dehydrogenation of the 4-en-3-oxo-derivative and 1.2-hydrogenation of the 1.4-dien-3-oxo-derivative. It was not possible to separate the hydrogenating activity from the dehydrogenating activity by affinity chromatography, disc electrophoresis, SDS-electrophoresis, and isoelectric focusing techniques. The pure enzyme preparation is discussed as only one FAD depending protein acting in response to the system as a dehydrogenase as well as a reductase.

Related Concepts

3-oxosteroid delta(1) dehydrogenase
Androstadienes
Androstenedione
NADH
NADP
Micropolyspora
Oxidase

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