Steroidal and gonadal effects on neural cell proliferation in vitro in an adult songbird

Brain Research
Anahid MirzatoniBarney A Schlinger

Abstract

Neurogenesis in the adult songbird brain occurs along the ventricular zone (VZ), a specialized cell layer surrounding the lateral ventricles. To examine the acute effects of sex steroids on VZ cell proliferation, male and female adult zebra finch brain slices containing the VZ were exposed to 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU) in vitro. Slices from one hemisphere served as the control, while contralateral slices were treated with steroids, steroidogenic enzyme inhibitors or gonadal tissue itself. There were no significant effects on VZ cell proliferation in either sexes by acute exposure to 17beta-estradiol (E2), dihydrotestosterone (DHT), a cocktail of four sex steroids, and inhibitors of sex steroid synthesis (aminoglutethimide, ketoconazole, and fadrozole), or by activation of a mitochondrial cholesterol transporter. By contrast, dehydroepiandrosterone (DHEA) suppressed VZ cell proliferation in males, but not females, replicating previous observations involving treatments with corticosterone and RU-486. This suggests that DHEA suppresses proliferation in males via a glucocorticoid receptor-related mechanism. These results suggest that neurosteroidogenesis per se has little effect on acute VZ cell proliferation. ...Continue Reading

References

Aug 21, 1992·Brain Research. Developmental Brain Research·S A Goldman, M Nedergaard
Nov 1, 1991·The Journal of Endocrinology·S G Hillier
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E DiCicco-Bloom, I B Black
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C CorpéchotE E Baulieu
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S A Goldman, F Nottebohm
Sep 11, 1995·The Journal of Comparative Neurology·P ShenA P Arnold
May 1, 1993·Journal of Neurobiology·S D Brown, S W Bottjer
Aug 26, 1998·Journal of Neurobiology·S A Goldman
Oct 6, 1998·Annual Review of Biochemistry·J Massagué
Nov 11, 1998·Nature Medicine·P S ErikssonF H Gage
May 14, 1999·Trends in Cognitive Sciences·E GouldT J Shors
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·S ShimasakiG F Erickson
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·X C LiF Nottebohm
Aug 22, 2001·Neurochemical Research·J M TorresE Ortega
Dec 18, 2001·Brain Research. Brain Research Reviews·P AbsilJ Balthazart
Apr 16, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Sharon L RippRussell A Prough
May 29, 2002·Brain Research Bulletin·Jose Manuel García-VerdugoEnrique Font
Jun 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·A M GhoumariE-E Baulieu
Jul 31, 2003·Trends in Cell Biology·Marianna B Ruzinova, Robert Benezra
Oct 7, 2004·Brain Research. Developmental Brain Research·E A KellerM L Fiszman
Nov 24, 2004·The Journal of Comparative Neurology·Valerie Dewulf, Sarah W Bottjer
Aug 22, 2006·General and Comparative Endocrinology·Helen Tam, Barney A Schlinger
Aug 29, 2006·Endocrinology·Sarah E LondonBarney A Schlinger
Sep 8, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Linda WilbrechtFernando Nottebohm
Oct 31, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Sara SaponaroFrancesca Silvagno
Mar 31, 2007·The Journal of Comparative Neurology·Sarah E London, Barney A Schlinger

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