Steroidogenesis in vitro of human granulosa-luteal cells pretreated in vivo with gonadotropin-releasing hormone analogs

Fertility and Sterility
A Pellicer, F Miró

Abstract

A modulatory role for gonadotropin-releasing hormone (GnRH) on granulosa cell functions has been demonstrated in animals. Because human granulosa cells have specific receptors for GnRH, we have compared steroidogenesis in vitro of cells pretreated in vivo with GnRH analogs (GnRH-a) with other ovarian stimulation regimens used in in vitro fertilization (IVF) cycles. Cells from 13 patients treated with a GnRH-a for 26.8 +/- 1.1 days during pituitary desensitization and ovarian stimulation with gonadotropins were cultured. Their steroidogenesis was evaluated in basal conditions as well as after stimulation with follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hCG). Granulosa-luteal cells from 9 women treated with a combination of clomiphene citrate (CC) and gonadotropins were also cultured. Estradiol production in response to FSH was stimulated only in the GnRH-a-treated cells. Basal, FSH-, and hCG-induced progesterone (P) accumulation was higher in the CC-treated cells. The peak of P production was delayed 2 days in the GnRH-a-treated group. Only cells pretreated in vivo with GnRH-a accumulated 20 alpha-hydroxyprogesterone in culture. The results of the present study suggest that the use of GnRH-a during a lon...Continue Reading

Citations

Jul 23, 2008·The Cochrane Database of Systematic Reviews·Salim Daya, Joanne L Gunby
Apr 11, 2006·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Kamel Abdelhakim MohamedHany Lashen
Dec 20, 2007·Reproductive Sciences·Chryssa MetallinouNikos Nikolettos
Jun 1, 1992·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·F MiróA Pellicer
Oct 12, 2001·Annals of the New York Academy of Sciences·A TavaniotouP Devroey
Mar 30, 2001·Human Reproduction·O OrtmannK Diedrich
Sep 15, 2005·Journal of Neuroendocrinology·J P HapgoodK Ronacher
Oct 6, 2005·Reproductive Biology and Endocrinology : RB&E·Mohamed F M MitwallyMichael P Diamond

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