Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation

Clinics
Gisele Negro LimaJosé Maria Soares Júnior

Abstract

To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 µg/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip(®) Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip(®) Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of ...Continue Reading

Citations

Nov 1, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Ying HuangJie Qiao
Oct 4, 2017·Reproduction, Fertility, and Development·T FengL J Spicer
Feb 18, 2018·Journal of Pineal Research·Mei LanShao-Chen Sun
Apr 4, 2017·Reproduction, Fertility, and Development·Giuseppina BasiniFrancesca Grasselli
Jan 1, 2018·Reproduction, Fertility, and Development·José-Alfonso AbeciaAdriana Casao
Aug 8, 2019·Revista da Associação Médica Brasileira·Leonardo Augusto LombardiJosé Maria Soares

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Methods Mentioned

BETA
contraception
chip
PCR

Software Mentioned

GeneChip
GeneChip Operating Software
Limma
GraphPad
GraphPad Prism
RankProd
Primer Express
MicroArray Suite
Affymetrix

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