Steroidogenic enzyme expression during preovulatory follicle maturation in pigs

Biology of Reproduction
W M Garrett, H D Guthrie

Abstract

During preovulatory maturation, follicles destined to ovulate exhibit a marked increase in steroidogenesis, while nonovulatory, steroidogenically inactive follicles are lost by atresia. The purpose of this study was to assess the expression of the steroidogenic enzymes cytochrome P450 aromatase (P450arom), cytochrome P450 17alpha-hydroxylase/C(17-20) lyase (P450c17), and 3beta hydroxysteroid dehydrogenase (3betaHSD) and that of the cell proliferation-associated nuclear antigen Ki-67 by immunohistochemistry in both nonatretic and atretic follicles developed during altrenogest-synchronized preovulatory maturation. Fifteen cyclic gilts were slaughtered on Days 1, 3, 5 (n = 3 per day), and 7 (n = 6) following the withdrawal of altrenogest. Staining intensity was assigned a numeric value and was related to follicle size and day following altrenogest withdrawal. Follicle atresia was assessed by the extent of in situ 3' end-labeling of DNA in apoptotic cells. The overall incidence of atresia was 41%, 17%, and 7% in small (< 3 mm), medium (3-5 mm), and large (> 5 mm) follicles, respectively. Expression of granulosa cell P450arom, Ki-67 antigen, and thecal P450c17 was significantly less (p < 0.001) in atretic compared to nonatretic foll...Continue Reading

Citations

Mar 4, 2000·Animal Reproduction Science·E L GregoraszczukA Gertler
Apr 4, 2003·Biology of Reproduction·C J CorbinA J Conley
Dec 10, 2009·Folia Histochemica Et Cytobiologica·Anna KozłowskaBarbara Jana
Sep 5, 2013·International Journal of Molecular Sciences·Martin Krøyer RasmussenGalia Zamaratskaia
Apr 22, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Dieter SchamsBajram Berisha
Mar 7, 2006·General and Comparative Endocrinology·Pedro MayorManel Lopez-Bejar
Jul 13, 2005·Domestic Animal Endocrinology·Murat YarimTarik Atmaca
Jan 16, 2007·Molecular Reproduction and Development·Marie Saint-DizierSylvie Chastant-Maillard

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