Steroids in the equine oviduct: synthesis, local concentrations and receptor expression

Reproduction, Fertility, and Development
Hilde NelisKatrien Smits

Abstract

Steroids play an important role in mammalian reproduction and early pregnancy. Although systemic changes in steroid concentrations have been well documented, it is not clear how these correlate with local steroid concentrations in the genital tract. We hypothesised that, in the horse, the preimplantation embryo may be subjected to high local steroid concentrations for several days. Therefore, we measured progesterone, 17-hydroxyprogesterone, 17?-oestradiol, testosterone and 17?-testosterone concentrations in equine oviductal tissue by ultra-HPLC coupled with tandem mass spectrometry, and progesterone, 17?-oestradiol, oestrone and testosterone concentrations in oviduct fluid by radioimmunoassay, with reference to cycle stage and side of ovulation. Progesterone concentrations were high in oviductal tissue and fluid ipsilateral to the ovulation side during dioestrus, whereas other steroid hormone concentrations were not influenced by the side of ovulation. These results suggest that the high ipsilateral progesterone concentration is caused by: (1) contributions from the follicular fluid in the oviduct and diffusion of follicular fluid steroids after ovulation; (2) local transfer of steroids via blood or lymph; (3) local synthesis ...Continue Reading

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Citations

Jun 6, 2016·Theriogenology·Julie LamyMarie Saint-Dizier
Jan 8, 2020·International Journal of Molecular Sciences·Marie Saint-DizierPascal Mermillod
Apr 15, 2020·Animals : an Open Access Journal From MDPI·Katiuska SatuéPietro Medica
Jun 1, 2021·Biology of Reproduction·Coline MahéMarie Saint-Dizier

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Methods Mentioned

BETA
reverse-phase chromatography
confocal microscopy

Software Mentioned

SPSS
BLAST
G Power
Cell F
Xcalibur
Protein BLAST
ImageJ
Excel

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