PMID: 11906921Mar 22, 2002Paper

Steroids modulate transepithelial resistance and Na(+) absorption across cultured porcine vas deferens epithelia

Biology of Reproduction
Maureen L Phillips, Bruce D Schultz

Abstract

Epithelial cells were isolated from adult porcine vas deferens and grown in the absence or presence of steroid hormones. Transepithelial resistance (R(te)), basal short circuit current (I(sc)), and the effects of selected ion transport modulators on these parameters were evaluated in modified Ussing chambers at three time points (5-8, 11-14, and 18-22 days postseeding). At the earliest time point, no significant differences were observed. At the middle time point, when compared with R(te) in untreated control monolayers, R(te) in monolayers exposed to 17beta-estradiol, aldosterone, cortisol, cortisone, prednisolone, prednisone, and dexamethasone was significantly lower; in contrast, R(te) in monolayers exposed to testosterone, dihydrotestosterone, or progesterone did not differ from that in control monolayers. Treatments with cortisol, prednisolone, and dexamethasone were associated with an elevated basal I(sc) that was amiloride sensitive, indicating ongoing Na(+) absorption by these monolayers. R(te) was increased by amiloride treatment in glucocorticoid-treated monolayers but remained significantly less than that of control monolayers. At the third time point, the postamiloride R(te) of glucocorticoid-treated monolayers was ...Continue Reading

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Citations

Jun 15, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Kristen J Navara
Feb 27, 2003·Biology of Reproduction·Ryan W CarlinBruce D Schultz
May 29, 2009·Biology of Reproduction·Fernando Pierucci-AlvesBruce D Schultz
Aug 18, 2009·Biology of Reproduction·Fernando Pierucci-AlvesBruce D Schultz
May 20, 2011·Biology of Reproduction·Fernando Pierucci-AlvesBruce D Schultz
Jun 25, 2010·Molecular and Cellular Endocrinology·Erick J R SilvaMaria Christina W Avellar
Jun 9, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Victoria SharpAnthony E Michael
Oct 21, 2004·American Journal of Reproductive Immunology : AJRI·Katherine S Grant-Tschudy, Charles R Wira
Sep 13, 2002·American Journal of Physiology. Cell Physiology·Ryan W CarlinBruce D Schultz

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