Sterol 27-hydroxylase- and apoAI/phospholipid-mediated efflux of cholesterol from cholesterol-laden macrophages: evidence for an inverse relation between the two mechanisms

Arteriosclerosis, Thrombosis, and Vascular Biology
J WestmanU Diczfalusy

Abstract

Cholesterol-laden, human monocyte-derived macrophages were found to contain 27-hydroxycholesterol in proportion to their content of cholesterol ester. In accordance with previous work with human lung alveolar macrophages, there was a significant efflux of 27-hydroxycholesterol and 3beta-hydroxy-5-cholestenoic acid from the cultured cells. The efflux of 27-hydroxycholesterol was proportional to the cellular content of this steroid. Incubation of cholesterol-laden macrophages with reconstituted discoidal complexes made from apolipoprotein A-I and phospholipids resulted in a decrease in total cellular cholesterol, an increase in the efflux of free cholesterol, and a concomitant decrease in the total production and efflux of 27-oxygenated steroids, in particular, 3beta-hydroxy-5-cholestenoic acid. Reconstituted discoidal complexes with the Milano variant of apolipoprotein A-I gave virtually identical results, whereas high density lipoprotein was less efficient. These results suggest that cultured cholesterol-laden cells can export some of their excess cholesterol in the form of 27-hydroxycholesterol, 3beta-hydroxy-5-cholestenoic acid, and free cholesterol. In the presence of exogenous cholesterol acceptors, export of free cholester...Continue Reading

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S K BasuM S Brown
Oct 1, 1978·Analytical Biochemistry·A Hara, N S Radin
Jun 1, 1976·Scandinavian Journal of Immunology·A Bøyum
Jan 1, 1986·Methods in Enzymology·M C Cheung
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·I BjörkhemE Lund
Jul 26, 1996·The Journal of Biological Chemistry·I C GelissenW Jessup
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON
Jun 1, 1996·Trends in Cell Biology·Y Lange, T L Steck

❮ Previous
Next ❯

Citations

Mar 20, 2004·Trends in Cardiovascular Medicine·Daniel S Ory
May 18, 1999·Current Opinion in Lipidology·I BjörkhemD Lütjohann
Mar 29, 2014·Cholesterol·Joshua S WootenRon C Hoogeveen
Oct 4, 2006·Physiological Reviews·Elina Ikonen
Jan 5, 2000·Physiological Reviews·G J Schroepfer
Jan 18, 2003·The Journal of Biological Chemistry·Genevieve EscherDmitri Sviridov
Aug 16, 2001·The Journal of Biological Chemistry·X FuE G Lund
Sep 15, 2001·The Journal of Biological Chemistry·H VosperC N Palmer
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·E HallW Pandak
Jul 14, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Irundika Hk DiasPablo Martinez-Lage
Jun 17, 2000·Biochimica Et Biophysica Acta·L B Agellon, E C Torchia
Jan 17, 2012·Trends in Endocrinology and Metabolism : TEM·Agata A BielskaDaniel S Ory

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.