Sterol Carrier Protein 2, a Critical Host Factor for Dengue Virus Infection, Alters the Cholesterol Distribution in Mosquito Aag2 Cells

Journal of Medical Entomology
Qiang FuQue Lan

Abstract

Host factors that enable dengue virus (DENV) to propagate in the mosquito host cells are unclear. It is known that cellular cholesterol plays an important role in the life cycle of DENV in human host cells but unknown if the lipid requirements differ for mosquito versus mammalian. In mosquito Aedes aegypti, sterol carrier protein 2 (SCP-2) is critical for cellular cholesterol homeostasis. In this study, we identified SCP-2 as a critical host factor for DENV production in mosquito Aag2 cells. Treatment with a small molecule commonly referred to as SCPI-1, (N-(4-{[4-(3,4-dichlorophenyl)-1,3-thiazol-2-yl]amino}phenyl)acetamide hydrobromide, a known inhibitor of SCP-2, or knockdown of SCP-2 dramatically repressed the virus production in mosquito but not mammalian cells. We showed that the intracellular cholesterol distribution in mosquito cells was altered by SCP-2 inhibitor treatment, suggesting that SCP-2-mediated cholesterol trafficking pathway is important for DENV viral production. A comparison of the effect of SCP-2 on mosquito and human cells suggests that SCPI-1 treatment decreases cholesterol in both cell lines, but this decrease in cholesterol only leads to a decline in viral titer in mosquito host cells, perhaps, owing t...Continue Reading

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Citations

Nov 5, 2016·Progress in Lipid Research·Miguel A Martín-AcebesJuan-Carlos Saiz
Jan 13, 2017·PLoS Neglected Tropical Diseases·Natapong JupatanakulGeorge Dimopoulos
Dec 29, 2017·Parasites & Vectors·Gerard TerradasElizabeth A McGraw
Aug 6, 2019·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Hirunika Perera, Tharaka Wijerathna
Sep 20, 2020·Communications Biology·Cassandra KohElizabeth A McGraw
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Mar 20, 2021·Frontiers in Physiology·Octavio A C TalyuliGabriela O Paiva-Silva
Jun 13, 2021·Communications Biology·Christine M ReitmayerCourtney C Murdock

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