Sterol carrier protein-2, a new fatty acyl coenzyme A-binding protein.

The Journal of Biological Chemistry
A FrolovF Schroeder

Abstract

The ability of sterol carrier protein-2 (SCP-2) to interact with long chain fatty acyl-CoAs was examined. SCP-2 bound fluorescent fatty acyl-CoAs at a single site with high affinity. Kd values for cis- and trans-parinaroyl-CoA were 4.5 and 2.8 nM, respectively. Saturated 10-18-carbon and unsaturated 14-20-carbon fatty acyl-CoAs displaced SCP-2-bound fluorescent ligand. Oleoyl-CoA and oleic acid (but not coenzyme A) significantly altered SCP-2 Trp50 emission and anisotropy decay, thereby increasing SCP-2 rotational correlation time, SCP-2 hydrodynamic radius, and SCP-2 Trp50 remaining anisotropy up to 1.7-, 1.2-, and 1.3-fold, respectively. These changes were not accompanied by significant alterations in protein secondary structure as determined by circular dichroism. Finally, SCP-2 differentially altered the fluorescence emission and anisotropy decays of bound cis- and trans-parinaroyl-CoA. Both fluorescent fatty acyl-CoAs were located within a very ordered (limited cone angle of rotation) environment within SCP-2, as shown by a remaining anisotropy of 0.365 and 0.361 and a wobbling cone angle of 12 and 13 degrees , respectively. These anisotropy values were very close to those of such ligands in a propylene glass. However, the...Continue Reading

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Citations

May 5, 2011·Molecular and Cellular Biochemistry·Heather A HostetlerFriedhelm Schroeder
Jun 10, 2008·Lipids·Avery L McIntoshFriedhelm Schroeder
Jul 3, 2002·Chemistry and Physics of Lipids·Adalberto M GallegosFriedhelm Schroeder
Jun 19, 1998·Chemistry and Physics of Lipids·F SchroederA Frolov
Oct 10, 2001·Progress in Lipid Research·A M GallegosF Schroeder
Jul 17, 1999·Biochimica Et Biophysica Acta·C JatzkeG Assmann
Apr 15, 1997·European Journal of Biochemistry·M HummelA Hasilik
Aug 4, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Stephen M StoreyFriedhelm Schroeder
Mar 31, 2005·Seminars in Cell & Developmental Biology·Annette L Henneberry, Stephen L Sturley
Jan 10, 2016·Biochimica Et Biophysica Acta·Shiu-Cheung Lung, Mee-Len Chye
Apr 7, 2010·Progress in Lipid Research·Sonia GulatiStephen L Sturley
Jun 5, 2007·Biochimica Et Biophysica Acta·Friedhelm SchroederAnn B Kier
Jan 20, 2015·Journal of Neurochemistry·Khalil BouyakdanThierry Alquier
Jun 28, 2015·Archives of Biochemistry and Biophysics·Gregory G MartinAnn B Kier
Dec 13, 2006·Molecular Cell·Will A StanleyMatthias Wilmanns
May 12, 2016·Journal of Neurochemistry·Gregory G MartinFriedhelm Schroeder
Aug 24, 2016·Biochemistry·Huan HuangFriedhelm Schroeder
Nov 20, 2016·Journal of Neurochemistry·Gregory G MartinFriedhelm Schroeder
Apr 16, 2017·Journal of Lipid Research·Stephen M StoreyFriedhelm Schroeder
Oct 18, 2003·Journal of Lipid Research·Tobias B DansenKarel W A Wirtz
Mar 3, 2004·Journal of Lipid Research·Barbara P AtshavesFriedhelm Schroeder

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