Sterol regulation of 3-hydroxy-3-methylglutaryl-coenzyme A synthase gene through a direct interaction between sterol regulatory element binding protein and the trimeric CCAAT-binding factor/nuclear factor Y.

The Journal of Biological Chemistry
K A DooleyT F Osborne

Abstract

3-Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase, a key regulatory enzyme in the pathway for endogenous cholesterol synthesis, is a target for negative feedback regulation by cholesterol. The promoter for HMG-CoA synthase contains two binding sites for the sterol regulatory element-binding proteins (SREBPs). When cellular sterol levels are low, the SREBPs are released from the endoplasmic reticulum membrane, allowing them to translocate to the nucleus and activate SREBP target genes. In all SREBP-regulated promoters studied to date, additional co-regulatory transcription factors are required. In the HMG-CoA synthase promoter there are several potential co-regulatory transcription factor binding sites, including an inverted CCAAT box. A similar element has been shown to function with SREBP to mediate sterol regulation of another gene involved in cholesterol metabolism, farnesyl diphosphate synthase. Here, we show that CCAAT binding factor/nuclear factor Y (CBF/NF-Y) binding to the CCAAT box is required for sterol-regulated transcription of HMG-CoA synthase. The SREBP sites and the inverted CCAAT box are normally separated by 17 base pairs, and we show that increasing this distance results in a decrease in the level of tr...Continue Reading

References

Jan 1, 1989·Annual Review of Biochemistry·P F Johnson, S L McKnight
Jul 29, 1988·Science·S N MaityB de Crombrugghe
Jan 1, 1983·Methods in Enzymology·J L GoldsteinM S Brown
Sep 15, 1995·The Journal of Biological Chemistry·S M JacksonP A Edwards
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S SinhaB de Crombrugghe
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·X HuaX Wang
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·J EricssonP A Edwards
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·J M LopezT F Osborne
Oct 25, 1996·The Journal of Biological Chemistry·R SatoM Maeda

❮ Previous
Next ❯

Citations

Jun 13, 2006·Cellular and Molecular Neurobiology·Pelin Kelicen, Agneta Nordberg
May 10, 2013·Archives of Pharmacal Research·Chia-Hsin HuangHorng-Mo Lee
Nov 14, 2000·The American Journal of Cardiology·W C Cromwell, P E Ziajka
Jun 27, 2002·Biochemical and Biophysical Research Communications·Masaaki NagaiTeruo Ono
May 4, 2000·Progress in Lipid Research·N D RidgwayM K Storey
Feb 24, 2000·Biochimica Et Biophysica Acta·J ZhangA Fine
Dec 9, 2000·Biochimica Et Biophysica Acta·T R Tansey, I Shechter
Dec 9, 2000·Biochimica Et Biophysica Acta·P A EdwardsA Venkateswaran
Dec 9, 2000·Biochimica Et Biophysica Acta·K SchoonjansJ Auwerx
Sep 15, 2009·The Journal of Biological Chemistry·Christian BachIna Vorberg
Aug 31, 2010·DNA and Cell Biology·Alex K LeeSandra D O'Dell
Jan 2, 2003·The Plant Cell·Raymond W KwongJohn J Harada
Sep 2, 2004·Molecular and Cellular Biology·Julia I TothTimothy F Osborne
Jul 14, 2005·Annual Review of Nutrition·Harini Sampath, James M Ntambi
Jun 6, 2009·Journal of Carcinogenesis·Yumiko Yasui, Takuji Tanaka
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·S XiongS J Wakil
Dec 7, 2013·BioMed Research International·Xiaoyun WuBin Liang
Aug 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Maria T Bengoechea-AlonsoJohan Ericsson
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J N Athanikar, T F Osborne
Jul 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Guoxun ChenMichael S Brown
Jul 9, 2014·Cell Reports·Xiaoying WuJared Rutter
Dec 2, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Jia LiuLu-Qi Huang
Mar 3, 2001·Neoplasia : an International Journal for Oncology Research·J Q ZhaoW N Keith
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Orna Ben-NaimEliezer Lifschitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.