PMID: 490047Jul 1, 1979Paper

Sterol synthesis. High-pressure liquid chromatography of C27 sterol precursors of cholesterol.

Journal of Lipid Research
J R Thowsen, G J Schroepfer

Abstract

Conditions have been described which permit the rapid (approximately 3.5 hr) column chromatographic separation of the acetate derivatives of a number of C27 sterol precursors of cholesterol differing only in the number and position of double bonds in the sterol nucleus. On columns containing muPorasil with hexane-benzene 9:1 as the eluting solvent, acetate derivatives of the delta5, delta8(14), delta8, delta7, delta8,7, delta 8,14, and delta7,14 sterols were separated.

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