PMID: 9537392Apr 16, 1998Paper

Sterol uptake in Saccharomyces cerevisiae heme auxotrophic mutants is affected by ergosterol and oleate but not by palmitoleate or by sterol esterification

Journal of Bacteriology
F NessE Degryse

Abstract

The relationship between sterol uptake and heme competence in two yeast strains impaired in heme synthesis, namely, G204 and H12-6A, was analyzed. To evaluate heme availability, a heterologous 17alpha-hydroxylase cytochrome P-450 cDNA (P-450c17) was expressed in these strains, and its activity was measured in vivo. Heme deficiency in G204 led to accumulation of squalene and lethality. The heterologous cytochrome P-450 was inactive in this strain. The leaky H12-6A strain presented a slightly modified sterol content compared to that for the wild type, and the P-450c17 recovered partial activity. By analyzing sterol transfer on nongrowing cells, it was shown that the cells were permeable toward exogenous cholesterol when they were depleted of endogenous sterols, which was the case for G204 but not for H12-6A. It was concluded that the fully blocked heme mutant (G204) replenishes its diminishing endogenous sterol levels during growth by replacement with sterol from the outside medium. Endogenous sterol biosynthesis appears to be the primary factor capable of excluding exogenous sterol. Oleate but not palmitoleate was identified as a component that reduced but did not prevent sterol transfer. Sterol transfer was only slightly affect...Continue Reading

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Citations

Aug 14, 2019·Applied Microbiology and Biotechnology·Catherine Tesnière
Jun 13, 2020·Antibiotics·Somanon BhattacharyaBettina C Fries
Jun 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·David A HannaAmit R Reddi
Jan 7, 2003·Applied and Environmental Microbiology·Eric RosenfeldJean-Michel Salmon
Dec 29, 2006·Yeast·I S Ishtar Snoek, H Yde Steensma
Dec 16, 2003·Journal of Dairy Science·E I PsomasN Tzanetakis
Feb 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Paul F SouthScott D Briggs
Jan 25, 2005·Applied Microbiology and Biotechnology·Christelle DeytieuxJean-Michel Salmon
Jun 21, 2018·The Journal of Biological Chemistry·David A HannaAmit R Reddi
Jul 18, 2018·The Journal of Biological Chemistry·Elizabeth A SweenyDennis J Stuehr
Nov 3, 2004·Biochimica Et Biophysica Acta·Harald Pichler, Howard Riezman
Apr 17, 2007·Biochimica Et Biophysica Acta·Timothy A Schulz, William A Prinz

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