Sterols regulate endocytic pathways during flg22-induced defense responses in Arabidopsis

Development
Yaning CuiJinxing Lin

Abstract

The plant transmembrane receptor kinase FLAGELLIN SENSING 2 (FLS2) is crucial for innate immunity. Although previous studies have reported FLS2-mediated signal transduction and endocytosis via the clathrin-mediated pathway, whether additional endocytic pathways affect FLS2-mediated defense responses remains unclear. Here, we show that the Arabidopsis thaliana sterol-deficient mutant steroid methyltransferase 1 displays defects in immune responses induced by the flagellin-derived peptide flg22. Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) coupled with single-particle tracking showed that the spatiotemporal dynamics of FLS2-GFP changed on a millisecond time scale and that the FLS2-GFP dwell time at the plasma membrane increased in cells treated with a sterol-extracting reagent when compared with untreated counterparts. We further demonstrate that flg22-induced FLS2 clustering and endocytosis involves the sterol-associated endocytic pathway, which is distinct from the clathrin-mediated pathway. Moreover, flg22 enhanced the colocalization of FLS2-GFP with the membrane microdomain marker Flot 1-mCherry and FLS2 endocytosis via the sterol-associated pathway. This indicates that plants may respond to pat...Continue Reading

References

Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·G FelixT Boller
Jun 15, 2000·The Plant Cell·A C DienerG R Fink
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·K Simons, D Toomre
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Mar 3, 2006·Genes & Development·Silke RobatzekThomas Boller
Mar 21, 2007·Nature Methods·Maximilian H Ulbrich, Ehud Y Isacoff
May 16, 2007·Chembiochem : a European Journal of Chemical Biology·Somes K DasHagan Bayley
Jul 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Antje HeeseJohn P Rathjen
Mar 26, 2009·Annual Review of Biochemistry·Gary J Doherty, Harvey T McMahon
Jun 16, 2009·Molecular & Cellular Proteomics : MCP·Thomas StanislasFrançoise Simon-Plas
Oct 24, 2009·Current Opinion in Plant Biology·Niloufer G Irani, Eugenia Russinova
Jun 30, 2010·Nature Reviews. Genetics·Peter N Dodds, John P Rathjen
Oct 14, 2010·The Journal of Biological Chemistry·Erik G HofmanPaul M P van Bergen en Henegouwen
Dec 2, 2010·Proceedings of the National Academy of Sciences of the United States of America·Satoshi NaramotoJirí Friml
Jan 11, 2011·Current Opinion in Microbiology·Cécile Segonzac, Cyril Zipfel
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Cristian H DannaFrederick M Ausubel
Oct 25, 2011·Nature Structural & Molecular Biology·Shalini T Low-NamDiane S Lidke
Nov 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Youssef BelkhadirJoanne Chory
Nov 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Catherine AlbrechtCyril Zipfel
May 5, 2012·Progress in Lipid Research·Jean-Luc CacasSébastien Mongrand
May 9, 2012·Nature Chemical Biology·Niloufer G IraniEugenia Russinova
May 12, 2012·Methods in Molecular Biology·Yajie Niu, Jen Sheen
Jun 19, 2012·Current Opinion in Plant Biology·Jacqueline Monaghan, Cyril Zipfel
Aug 30, 2012·The Journal of Biological Chemistry·Hugo McGuireRikard Blunck
Oct 18, 2012·The Plant Journal : for Cell and Molecular Biology·Michaela KopischkeSabine Rosahl
Apr 19, 2013·Frontiers in Plant Science·Koste A YadetaGitta Coaker
Apr 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yunlong DuJirí Friml

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Citations

Feb 29, 2020·International Journal of Molecular Sciences·Yangyang CaoRuili Li
Feb 23, 2020·The New Phytologist·Haiyan ZhangJinxing Lin
Jul 1, 2020·Frontiers in Plant Science·Congcong HuangYangrong Cao
Aug 7, 2019·Annual Review of Phytopathology·Gayani EkanayakeAntje Heese
Aug 20, 2019·Cellular and Molecular Life Sciences : CMLS·Meng YuJinxing Lin
Dec 15, 2020·Trends in Plant Science·Jingjing XingJinxing Lin
May 18, 2021·Frontiers in Plant Science·Tomáš TakáčJozef Šamaj
Jun 3, 2021·International Journal of Molecular Sciences·Ai-Yu GuoWen-Qiang Wu

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