Stevia Prevents Acute and Chronic Liver Injury Induced by Carbon Tetrachloride by Blocking Oxidative Stress through Nrf2 Upregulation

Oxidative Medicine and Cellular Longevity
Erika Ramos-TovarPablo Muriel

Abstract

The effect of stevia on liver cirrhosis has not been previously investigated. In the present study, the antioxidant and anti-inflammatory properties of stevia leaves were studied in male Wistar rats with carbon tetrachloride- (CCl4-) induced acute and chronic liver damage. Acute and chronic liver damage induced oxidative stress, necrosis, and cholestasis, which were significantly ameliorated by stevia. Chronic CCl4 treatment resulted in liver cirrhosis, as evidenced by nodules of hepatocytes surrounded by thick bands of collagen and distortion of the hepatic architecture, and stevia significantly prevented these alterations. Subsequently, the underlying mechanism of action of the plant was analyzed. Our study for the first time shows that stevia upregulated Nrf2, thereby counteracting oxidative stress, and prevented necrosis and cholestasis through modulation of the main proinflammatory cytokines via NF-κB inhibition. These multitarget mechanisms led to the prevention of experimental cirrhosis. Given the reasonable safety profile of stevia, our results indicated that it may be useful for the clinical treatment of acute and chronic liver diseases.

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Citations

Dec 19, 2018·Basic & Clinical Pharmacology & Toxicology·Sael Casas-GrajalesPablo Muriel
Nov 30, 2019·Archives of Pharmacal Research·Keiko Taguchi, Thomas W Kensler
Oct 20, 2018·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Erika Ramos-TovarPablo Muriel
Feb 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Asad UllahSyed Lal Badshah
Jan 3, 2020·European Journal of Gastroenterology & Hepatology·Eduardo E Vargas-Pozada, Pablo Muriel
Oct 10, 2020·Journal of Basic and Clinical Physiology and Pharmacology·Abeer F MostafaMona G Elhadidy
Dec 19, 2020·Antioxidants·Erika Ramos-Tovar, Pablo Muriel
Apr 2, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Jianxia WenYanling Zhao
Jul 20, 2021·European Journal of Pharmacology·Yu-Xin ZhangJi-Hua Fu
Jul 10, 2021·International Journal of Immunopathology and Pharmacology·Fei ZengZhengyuan Xia

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Methods Mentioned

BETA
Protein Assay
electrophoresis
Reverse Transcription Polymerase
PCR
MDA

Software Mentioned

ImageJ
Stevia
StepOne
GraphPad Prism®
ImageJ®

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