Stiff substrates increase YAP-signaling-mediated matrix metalloproteinase-7 expression

Oncogenesis
A NukudaH Haga

Abstract

Abnormally stiff substrates have been shown to trigger cancer progression. However, the detailed molecular mechanisms underlying this trigger are not clear. In this study, we cultured T84 human colorectal cancer cells on plastic dishes to create a stiff substrate or on collagen-I gel to create a soft substrate. The stiff substrate enhanced the expression of matrix metalloproteinase-7 (MMP-7), an indicator of poor prognosis. In addition, we used polyacrylamide gels (2, 67 and 126 kPa) so that the MMP-7 expression on the 126-kPa gel was higher compared with that on the 2-kPa gel. Next, we investigated whether yes-associated protein (YAP) affected the MMP-7 expression. YAP knockdown decreased MMP-7 expression. Treatment with inhibitors of epidermal growth factor receptor (EGFR) and myosin regulatory light chain (MRLC) and integrin-α2 or integrin-β1 knockdown downregulated MMP-7 expression. Finally, we demonstrated that YAP, EGFR, integrin-α2β1 and MRLC produced a positive feedback loop that enhanced MMP-7 expression. These findings suggest that stiff substrates enhanced colorectal cancer cell viability by upregulating MMP-7 expression through a positive feedback loop.

References

Apr 10, 1995·International Journal of Cancer. Journal International Du Cancer·H YamamotoK Imai
Feb 1, 1996·The International Journal of Biochemistry & Cell Biology·C L Wilson, L M Matrisian
Apr 1, 1997·Journal of Mammary Gland Biology and Neoplasia·S B Fox, A L Harris
Jan 26, 2002·Cancer Letters·Nailin HuoHiroshi Shimada
May 26, 2004·Biochemical Society Transactions·S CabodiP Defilippi
Dec 15, 2004·Biophysical Journal·Hisashi HagaKazushige Kawabata
Mar 12, 2005·The Journal of Biological Chemistry·Akira KatsumiMartin Alexander Schwartz
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Oct 12, 2005·The Journal of Cell Biology·Johan de RooijClare M Waterman-Storer
Dec 29, 2005·Experimental Biology and Medicine·Masanori IiYasuhisa Shinomura
Aug 23, 2006·Journal of Cellular Physiology·Takeomi MizutaniKazushige Kawabata
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Diana E Jaalouk, Jan Lammerding
Jan 6, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Selja KoskensaloCaj Haglund
May 17, 2011·Nature Cell Biology·Christophe GuilluyKeith Burridge
Jun 10, 2011·Nature·Sirio DupontStefano Piccolo
Oct 18, 2011·The Biochemical Journal·Ming-Cheng ChangWen-Fang Cheng
Aug 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·John M LamarRichard O Hynes
Jul 12, 2013·Journal of Human Hypertension·M PuatoP Pauletto
Oct 12, 2013·Experimental Cell Research·Seiichiro IshiharaHisashi Haga
Jun 24, 2014·Cell·Diane D ShaoWilliam C Hahn
Aug 3, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yinghai YeJun Fang

❮ Previous
Next ❯

Citations

Apr 30, 2016·Biochemical and Biophysical Research Communications·Akihiro NukudaHisashi Haga
May 6, 2016·Cellular and Molecular Life Sciences : CMLS·Joana F S PereiraPeter Jordan
Nov 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Yuxin LiuZhenan Bao
May 11, 2019·PloS One·Michael E BregenzerGeeta Mehta
May 17, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Ye DingMing-Hui Zou
Nov 21, 2019·Cells·Lauren Eric Swenarchuk
Feb 26, 2020·Bioengineering·Wataru SugimotoKeiko Kawauchi
Aug 23, 2020·International Journal of Oral Science·Mohit SharmaRaghu Radhakrishnan
Sep 5, 2020·Journal of Cellular Physiology·Yutong GuoJuan Li
Feb 23, 2020·Cells·Antonio PalumboLuiz Eurico Nasciutti
Mar 16, 2018·Frontiers in Cell and Developmental Biology·Josette M NorthcottValerie M Weaver
Jul 25, 2019·Scientific Reports·Raj Pranap ArunRama Shanker Verma
Apr 30, 2020·Biophysics Reviews·Tomoyasu AizawaMin Yao
May 30, 2020·Cells·Maryam Raeeszadeh-SarmazdehBrianne G Hritz
Oct 1, 2020·Development·Hannah K VanyaiBarry Thompson
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Juhyeon JoDong-Hwee Kim
Jun 17, 2020·Ageing Research Reviews·Alexander Fedintsev, Alexey Moskalev
Feb 23, 2021·Frontiers in Oncology·Aukie HooglugtStephan Huveneers
Aug 17, 2020·The Journal of Physical Chemistry Letters·Fang TianHyokeun Park
Aug 12, 2019·ACS Biomaterials Science & Engineering·GeonHui LeeDong-Hwee Kim

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.