PMID: 2124584Oct 1, 1990Paper

Stiffened erythrocytes augment the pulmonary hemodynamic response to hypoxia

Journal of Applied Physiology
M P Doyle, B R Walker

Abstract

Isolated rat lungs were perfused with suspensions containing normal and stiffened erythrocytes (RBCs) during normoxic and hypoxic ventilation to assess the effect of reduced RBC deformability on the hypoxic pressor response. RBC suspensions were prepared with cells previously incubated in isotonic phosphate-buffered saline with or without 0.0125% glutaraldehyde. The washed RBCs were resuspended in isotonic bicarbonate-buffered saline (with 4% albumin) to hematocrits of approximately 35%. The lungs were perfused with control and experimental cell suspensions in succession while pulmonary arterial pressure was measured during normoxic (21% O2) and hypoxic (3% O2) ventilation. On the attainment of a peak hypoxic pressor response, flow rate was changed so that pressure-flow curves could be constructed for each suspension. RBC deformability was quantified by a filtration technique using 4.7-microns-pore filters. Glutaraldehyde treatment produced a 10% decrease in RBC deformability (P less than 0.05). Over the range of flow rates, Ppa was increased by 15-17% (P less than 0.05) and 26-31% (P less than 0.05) during normoxic and hypoxic ventilation, respectively, when stiffened cells were suspended in the perfusate. The magnitude of the...Continue Reading

Citations

Sep 11, 2007·Critical Care Medicine·Philip C SpinellaUNKNOWN 31st Combat Support Hospital Research Working Group
Jul 23, 2016·Clinical Hemorheology and Microcirculation·Hwang-Woon MoonSang-Seok Nam
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·B R WalkerL D Nelin
Jun 30, 2012·Sports Medicine·Robert A JacobsMax Gassmann
Sep 1, 1992·British Poultry Science·M H MaxwellC C McCorquodale
Mar 24, 2004·Journal of Intensive Care Medicine·Elhanan NahumTommy Schonfeld
May 4, 2021·Clinical Hemorheology and Microcirculation·Michael Buono, Kaitlyn Rostomily
Jan 9, 2003·Journal of the American College of Surgeons·Ernest E Moore

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