Stiffness of carbodiimide-crosslinked glycerinated muscle fibres in rigor and relaxing solutions at high salt concentrations

Journal of Muscle Research and Cell Motility
K Tawada, M Kimura

Abstract

In this article, we have applied a crosslinking technique with a water-soluble carbodiimide to single glycerol-extracted muscle fibres from the rabbit. We have measured the stiffness of the fibres in a relaxing solution at high salt concentration. These fibres were crosslinked to varying extents in the rigor state. The relaxing solution caused uncrosslinked crossbridge heads (S1) to detach. High salt concentrations were used because the fibres were not activated by the crosslinked crossbridges under these conditions, although they were at physiological ionic strength. We found a linear correlation between the extent of S1 crosslinking to thin filaments and the stiffness and that the stiffness in the relaxing solution of muscle fibres with all the S1 heads crosslinked to thin filaments was the same as the rigor stiffness of the fibres before crosslinking. We conclude that the sarcomere compliance is mostly a property of the crossbridges (with more than 65% of the crossbridge compliance in the S1 portions and less than 35% in the S2 portion) and little of other sarcomere structures. In an earlier paper [Kimura & Tawada, Biophys. J. 45, 603-10 (1984)], we demonstrated that the S2 portion of the crossbridge was stiff. It then follo...Continue Reading

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Citations

Mar 30, 2005·Journal of Biomaterials Science. Polymer Edition·Akira KakugoYoshihito Osada
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Mark S MillerJim O Vigoreaux
Feb 1, 1993·Journal of Muscle Research and Cell Motility·A Månsson
Mar 3, 2011·Journal of Biomedical Optics·Ali ShuaibGang Yao
Jul 3, 1995·Physical Review Letters·K Sekimoto, K Tawada
Oct 24, 1998·Biochimica Et Biophysica Acta·W X Li, M Schoenberg
Nov 20, 1988·Journal of Molecular Biology·M C ReedyR T Tregear

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