STIL is required for centriole duplication in human cells

Journal of Cell Science
Julia VulprechtAlwin Krämer

Abstract

Centrioles are key structural elements of centrosomes and primary cilia. In mammals, only a few proteins including PLK4, CPAP (CENPJ), SAS6, CEP192, CEP152 and CEP135 have thus far been identified to be required for centriole duplication. STIL (SCL/TAL1 interrupting locus, also known as SIL) is a centrosomal protein that is essential for mouse and zebrafish embryonic development and mutated in primary microcephaly. Here, we show that STIL localizes to the pericentriolar material surrounding parental centrioles. Its overexpression results in excess centriole formation. siRNA-mediated depletion of STIL leads to loss of centrioles and abrogates PLK4-induced centriole overduplication. Additionally, we show that STIL is necessary for SAS6 recruitment to centrioles, suggesting that it is essential for daughter centriole formation, interacts with the centromere protein CPAP and rapidly shuttles between the cytoplasm and centrioles. Consistent with the requirement of centrioles for cilia formation, Stil(-/-) mouse embryonic fibroblasts lack primary cilia--a phenotype that can be reverted by restoration of STIL expression. These findings demonstrate that STIL is an essential component of the centriole replication machinery in mammalian ...Continue Reading

References

Sep 5, 2001·Nature Reviews. Molecular Cell Biology·S Doxsey
Jan 17, 2002·Current Opinion in Cell Biology·Michel Bornens
May 3, 2002·Leukemia·A KrämerA D Ho
Oct 2, 2002·Nature Genetics·Jacquelyn BondC Geoffrey Woods
Nov 5, 2002·Nature Reviews. Cancer·Erich A Nigg
Dec 7, 2002·Nature Genetics·Sridhar RamaswamyTodd R Golub
Feb 26, 2003·Cell·Matthew KirkhamAnthony A Hyman
Feb 12, 2004·Methods in Enzymology·Robert D PhairTom Misteli
Apr 8, 2004·Developmental Cell·Catherine A KempKevin F O'Connell
Jul 3, 2004·Nature Cell Biology·Marie DelattrePierre Gönczy
Dec 2, 2004·Developmental Cell·Alexander DammermannKaren Oegema
Mar 29, 2005·Nature Genetics·Jacquelyn BondC Geoffrey Woods
Apr 5, 2005·American Journal of Human Genetics·C Geoffrey WoodsWolfgang Enard
Jul 19, 2005·Molecular and Cellular Biology·Stefano CampanerIlan R Kirsch
Oct 26, 2005·Nature Cell Biology·Robert HabedanckErich A Nigg
Dec 6, 2005·Current Biology : CB·M Bettencourt-DiasD M Glover
Jul 4, 2006·Cell·Renata BastoJordan W Raff
Jul 11, 2006·Developmental Cell·Erica E DavisNicholas Katsanis
Sep 19, 2006·Current Biology : CB·Marie DelattrePierre Gönczy
Dec 1, 2006·Nature·Laurence PelletierThomas Müller-Reichert
Jan 25, 2007·Nature Reviews. Molecular Cell Biology·Jens Lüders, Tim Stearns
Apr 26, 2007·Cancer Research·Ayelet ErezShai Izraeli
May 17, 2007·Nature Reviews. Molecular Cell Biology·Mónica Bettencourt-Dias, David M Glover
Jun 20, 2007·Molecular and Cellular Biology·Kathleen L PfaffLeonard I Zon
Aug 8, 2007·Developmental Cell·Julia Kleylein-SohnErich A Nigg
Oct 24, 2007·The Journal of Cell Biology·Susanne GraserErich A Nigg
Jan 5, 2008·Science·Anita RauchAndré Reis
Jun 17, 2008·Cell·Renata BastoJordan W Raff
Feb 14, 2009·American Journal of Human Genetics·Arun KumarSusan H Blanton
Jun 9, 2009·Nature Cell Biology·Chieh-Ju C TangTang K Tang
Oct 24, 2009·Trends in Genetics : TIG·Gemma K Thornton, C Geoffrey Woods
Jan 20, 2010·The Journal of Biological Chemistry·Jayachandran GopalakrishnanTomer Avidor-Reiss

❮ Previous
Next ❯

Citations

Jun 14, 2012·Nature Reviews. Molecular Cell Biology·Pierre Gönczy
Nov 2, 2012·Molecular Biology of the Cell·Brian A BaylessChad G Pearson
Aug 13, 2013·PloS One·Nicola J BrownVincenzo Costanzo
Mar 5, 2014·The Journal of Cell Biology·Debora KellerPierre Gönczy
Jan 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xiangdong ZhengJay Gopalakrishnan
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Masafumi Hirono
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Elif Nur Fırat-Karalar, Tim Stearns
Dec 31, 2014·BioMed Research International·Marine Barbelanne, William Y Tsang
Jul 17, 2013·The Journal of Cell Biology·Yi-Nan LinTang K Tang
Aug 16, 2012·Cellular and Molecular Life Sciences : CMLS·Christopher W Brownlee, Gregory C Rogers
Jun 22, 2014·FEBS Letters·Erich A NiggChristian Arquint
Dec 4, 2013·The Journal of Cell Biology·Joo-Hee SirFanni Gergely
Jun 9, 2015·Molecular and Cellular Probes·Deborah J Morris-Rosendahl, Angela M Kaindl
Jul 16, 2014·Developmental Cell·Chii Shyang FongMeng-Fu Bryan Tsou
Dec 9, 2014·Cell Cycle·Ahuvit DavidAlwin Krämer
Jun 17, 2016·Molecular & Cellular Oncology·Hiroki FujitaNatsuko Chiba
Nov 7, 2012·Current Opinion in Cell Biology·Benjamin D Vitre, Don W Cleveland
Jul 19, 2015·ELife·Christian ArquintTimm Maier
Jun 24, 2015·The Journal of Cell Biology·Tyler C MoyerAndrew J Holland
Mar 13, 2014·Current Biology : CB·Elif Nur Firat-KaralarTim Stearns
Feb 22, 2014·Current Biology : CB·Véronique Marthiens, Renata Basto
Sep 12, 2013·Chemical Communications : Chem Comm·Hadar AmartelyAssaf Friedler
Feb 7, 2015·PloS One·Charlotte MoudenVéronique David
Aug 27, 2016·Nature Communications·Yuki TsuchiyaDaiju Kitagawa
Feb 22, 2012·Cancer Letters·Simon J AnderhubBettina Maier
Nov 22, 2016·Nature Communications·Yuichiro WatanabeShigeomi Shimizu
May 26, 2017·Molecular Biology of the Cell·Akshari GuptaDaiju Kitagawa
Mar 3, 2018·The Journal of Cell Biology·Tiffany A McLamarrahGregory C Rogers
May 18, 2016·Journal of Cell Science·Ching-Wen ChangTang K Tang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.