STIM1 is required for Ca2+ signaling during mammalian fertilization

Developmental Biology
Kiho LeeZoltan Machaty

Abstract

During fertilization in mammals, a series of oscillations in the oocyte's intracellular free Ca(2+) concentration is responsible for oocyte activation and stimulation of embryonic development. The oscillations are associated with influx of Ca(2+) across the plasma membrane that is probably triggered by the depletion of the intracellular stores, a mechanism known as store-operated Ca(2+) entry. Recently, STIM1 has been identified in oocytes as a key component of the machinery that generates the Ca(2+) influx after store depletion. In this study, the involvement of STIM1 in the sperm-induced Ca(2+) oscillations and its significance in supporting subsequent embryo development were investigated. Downregulation of STIM1 levels in pig oocytes by siRNA completely inhibited the repetitive Ca(2+) signal triggered by the fertilizing sperm. In addition, a significantly lower percentage of oocytes cleaved or formed blastocysts when STIM1 was downregulated prior to fertilization compared to the control groups. Restoring STIM1 levels after fertilization in such oocytes by means of mRNA injection could not rescue embryonic development that in most cases was arrested at the 2-cell stage. On the other hand, STIM1 overexpression prior to fertili...Continue Reading

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Citations

Mar 26, 2014·Cellular Reprogramming·Kiho LeeZoltan Machaty
Feb 20, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Chunmin Wang, Zoltan Machaty
Mar 8, 2013·Molecular Biology of the Cell·Banyoon CheonRafael A Fissore
Aug 15, 2014·Stem Cell Research·Yu-Rim KwonJong-Sun Kang
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Oct 11, 2015·Cell and Tissue Research·Zoltan Machaty
Sep 9, 2015·Human Reproduction Update·Marc YesteKevin Coward
Aug 1, 2013·Molecular Reproduction and Development·Junaid KashirStephen A Stricker
Aug 14, 2012·Molecular Reproduction and Development·Yi-Liang Miao, Carmen J Williams
Nov 20, 2012·Biochemical and Biophysical Research Communications·Tooru TakahashiHideki Shirakawa
Jan 22, 2014·Reproductive Biomedicine Online·Luna MaoFan Jin
Jun 13, 2015·Biology of Reproduction·Chunmin WangZoltan Machaty
Nov 11, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Minerva Ferrer-BuitragoBjörn Heindryckx
Aug 21, 2019·Cold Spring Harbor Perspectives in Biology·Takuya WakaiRafael A Fissore
Jul 17, 2020·Open Biology·Paula SteinCarmen J Williams
Jul 13, 2018·Frontiers in Cell and Developmental Biology·Ingrid CarvachoKhaled Machaca
Nov 2, 2018·International Journal of Molecular Sciences·Sudip PaudelMargaret Saha
Oct 17, 2018·Proceedings of the National Academy of Sciences of the United States of America·Miranda L BernhardtCarmen J Williams
Oct 21, 2015·Journal of Cell Science·Miranda L BernhardtCarmen J Williams
Oct 9, 2013·Journal of Cell Science·Takuya WakaiRafael A Fissore

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