Stimulated migration and penetration of vascular endothelial cells into poly (L-lactic acid) scaffolds under flow conditions

Biomaterials Research
Min-Ah KooJong-Chul Park

Abstract

The initial procedure of the development of engineered tissues is cell seeding into three-dimensional polymer scaffolds. However, it is hard to make the cells invade into scaffold due to the characteristic of pore and material. Electrospun poly (L-lactic acid) scaffold and flow perfusion system were used to overcome these seeding problems. Before starting the experiment, we set up the parallel plate chamber system to observe endothelial cell migration under flow condition. In individual cell migration model, human umbilical endothelial cells started to migrate in the direction of flow at 8 dyne/cm(2) and we observed the cytoskeleton alignment at 8 dyne/cm(2). This study has demonstrated the possibility to evaluate and analyze cell migration using the parallel plate chamber system and we may predict in vivo cell migration under flow condition based on these results. Also the flow perfusion system was established for the effective cell seeding into at three dimensional scaffolds. Moreover, shear stress induced by flow can enhance cell migration into PLLA scaffold that is in the form of cotton. Result indicated that cell penetration was achieved under flow condition better and more than under static condition throughout the matrix.

References

Jul 1, 1995·Physiological Reviews·P F Davies
Jul 1, 1994·Bio/technology·L E FreedR Langer
Jan 1, 1993·Journal of Biomedical Materials Research·L E FreedR Langer
May 14, 1993·Science·R Langer, J P Vacanti
Jan 1, 1997·Journal of Vascular and Interventional Radiology : JVIR·E A SpragueJ C Palmaz
Jun 13, 1998·Biotechnology Progress·G Vunjak-NovakovicL E Freed
Apr 20, 1999·The Journal of Clinical Investigation·S LiS Chien
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Song LiShu Chien
Apr 30, 2003·The Journal of Cell Biology·Beata Wojciak-Stothard, Anne J Ridley
Sep 17, 2005·Journal of Cellular Biochemistry·Song LiSteven Hsu
Sep 29, 2005·Biochemical and Biophysical Research Communications·Steve HsuSong Li
Oct 26, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Kazunori ShimizuHiroyuki Honda
Dec 29, 2009·Journal of Biomedicine & Biotechnology·Alexander M SailonStephen M Warren
Jan 21, 2010·Tissue Engineering. Part B, Reviews·Gustavo A VillalonaChristopher K Breuer

❮ Previous
Next ❯

Citations

Nov 22, 2015·Annals of Biomedical Engineering·Maggie A OstrowskiAlexander R Dunn
Jun 21, 2015·Cytotechnology·Ieva BružauskaitėEiva Bernotienė
Feb 2, 2016·Tissue Engineering and Regenerative Medicine·Im Geon JinSoon Jung Hwang
Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Valeria MastrulloPaola Campagnolo
Jun 3, 2021·Biomedicines·Jernej VajdaBoštjan Vihar
Jul 14, 2021·Journal of Biomechanical Engineering·Meghan E FallonMonica T Hinds

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPases

Software Mentioned

SPSS
Tomoro
ImageJ
CCP

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

The Annals of Thoracic Surgery
Pascal Maria Dohmen, Wolfgang Konertz
Methods in Molecular Biology
Gordana Vunjak-Novakovic, Milica Radisic
Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
A PaimI C Tessaro
© 2021 Meta ULC. All rights reserved