PMID: 9186577May 23, 1997Paper

Stimulated rapid expression in vitro for early detection of in vivo T-cell receptor mutations induced by radiation exposure

Mutation Research
N IshiokaS Kyoizumi

Abstract

The T-cell receptor (TCR) mutation assay for in vivo somatic mutations is a sensitive indicator of exposure to ionizing radiation. However, this assay cannot be immediately applied after radiation exposure because expression of a mutant phenotype may require as long as several months. In the present study, we eliminate this time lag by stimulating lymphocytes with a mitogen that can accelerate the turnover of TCR protein expression in T-cells. When lymphocytes obtained from healthy donors were irradiated with various doses of X-rays and cultured with human interleukin-2 after phytohemagglutinin (PHA) pulse stimulation, the mutant frequency (MF) of CD4+ T-cells increased dose dependently during the first 7 days, then decreased rapidly due to the growth disadvantage of mutant cells. This suggests that PHA stimulation can shorten the expression time of a mutant phenotype to within a week after radiation exposure. The relationship between radiation dose and TCR MF on the seventh day was best fitted by a linear-quadratic dose-response model. We applied this improved TCR mutation assay to gynecological cancer patients who received 5 days of localized radiotherapy, totaling about 10 Gy. The in vivo TCR MF in the patients did not chang...Continue Reading

References

Mar 1, 1988·Mutation Research·M JanatipourA A Morley

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Citations

Dec 29, 2013·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Blanca LaffonEduardo Pásaro
Jul 31, 2014·Radiation and Environmental Biophysics·Valentina L RybkinaAlexander V Zurochka
Nov 14, 1997·Mutation Research·S UmekiS Kyoizumi
Oct 30, 2007·The Journal of Toxicological Sciences·Naoki KunugitaToshiyuki Norimura
Jun 10, 2003·Journal of Radiation Research·Fumio KatoToshiyuki Norimura
May 26, 2016·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Blanca LaffonVanessa Valdiglesias

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