Stimulating angiogenesis mitigates the unloading-induced reduction in osteogenesis in early-stage bone repair in rats

Physiological Reports
Takeshi Matsumoto, Shota Sato

Abstract

Accelerating fracture healing during bed rest allows early mobilization and avoids prolonged fracture healing times. We tested the hypothesis that stimulating angiogenesis with deferoxamine (DFO) mitigates the unloading-induced reduction in early-stage bone repair. Rats aged 12 weeks were subjected to cortical drilling on their tibial diaphysis under anesthesia and treated with hindlimb unloading (HU), HU and DFO administration (DFOHU), or weight bearing (WB) for 5 or 10 days (HU5/10, DFOHU5/10, WB5/10; n = 8 per groups) until sacrifice for vascular casting with a zirconium dioxide-based contrast agent. Taking advantage of its absorption discontinuity at the K-absorption edge, vascular and bone images in the drill-hole defects were acquired by synchrotron radiation subtraction CT. Bone repair was reduced in HU rats. The bone volume fraction (B.Vf) was 88% smaller in HU5 and 42% smaller in HU10 than in WB5/10. The bone segment densities (B.Seg) were 97% smaller in HU5 and 141% larger in HU10 than in WB5/10, and bone thickness (B.Th) was 38% smaller in HU10 than in WB10. The vascular volume fraction (V.Vf) was 35% and the mean vessel diameter (V.D) was 13% smaller in HU10 than in WB10. When compared according to categorized vesse...Continue Reading

References

Mar 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S KeilaM Weinreb
Apr 1, 1997·Calcified Tissue International·C H KasperkR Ziegler
Apr 1, 1997·IEEE Transactions on Medical Imaging·F MaesP Suetens
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·R CaoY Cao
Nov 14, 2001·Reviews in Endocrine & Metabolic Disorders·G R MundyS Harris
Mar 16, 2002·Journal of Applied Physiology·Emily R Morey-Holton, Ruth K Globus
Jul 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·John StreetEllen H Filvaroff
Oct 18, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Zachary ThompsonJill A Helms
Nov 20, 2002·Ageing Research Reviews·Ray MarksJoseph M Lane
Apr 23, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Douglas C MooreMichael G Ehrlich
Nov 25, 2003·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Hosam K KamelRaymond G Hoffmann
Dec 4, 2003·Drug Discovery Today·Richard A D Carano, Ellen H Filvaroff
Feb 6, 2004·Joint, Bone, Spine : Revue Du Rhumatisme·Régis sseurChristian Marcelli
Feb 12, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Roberta MelchionnaMonica Napolitano
Feb 23, 2005·Nature·Florent ElefteriouGerard Karsenty
May 11, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Miyuki Sakuma-ZenkeToshitaka Nakamura
May 12, 2005·Trends in Cardiovascular Medicine·Daniel J Ceradini, Geoffrey C Gurtner
Jan 19, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Libby KindlePatricia Collin-Osdoby
Jun 6, 2007·The Journal of Clinical Investigation·Dwight A Towler
Oct 10, 2007·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M W Hamrick, S L Ferrari
Jan 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chao WanThomas L Clemens
Feb 16, 2008·Journal of Applied Physiology·Kyunghwa BaekSusan A Bloomfield
Feb 23, 2008·Journal of Applied Physiology·Katherine A RadekCharles E Wade
Apr 1, 2008·Biochemical and Biophysical Research Communications·Zhi-Hu QuKe-Rong Dai
Aug 12, 2008·Seminars in Cell & Developmental Biology·Aaron SchindelerDavid G Little
Sep 24, 2008·International Wound Journal·Ramin Mostofizadeh Farahani, Luisa A DiPietro
Dec 31, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kyunghwa Baek, Susan A Bloomfield
Feb 17, 2009·Developmental Cell·Peter FraislPeter Carmeliet
Apr 1, 2009·Journal of Cellular Physiology·H H HuangR S Mason
Apr 2, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xing ShenShawn R Gilbert
Jun 30, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ernestina SchipaniGregg L Semenza
Dec 3, 2009·Calcified Tissue International·Laurent MonfouletJean-Christophe Fricain
Sep 8, 2010·Bone·Michael DoubeSandra J Shefelbine
Jul 9, 2011·Journal of Orthopaedic Trauma·Rena StewartShawn Gilbert
Jul 28, 2011·Journal of Cellular Biochemistry·Grace L Lin, Kurt D Hankenson
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Joel D BoerckelRobert E Guldberg
Dec 16, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Joel D BoerckelRobert E Guldberg

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Citations

Oct 24, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Allison W HsiaNicole M Collette
Nov 23, 2018·Calcified Tissue International·Vimal VeeriahMattia Capulli
Jul 1, 2017·Medical & Biological Engineering & Computing·Takeshi Matsumoto, Daichi Goto
Aug 7, 2016·Journal of Tissue Engineering·Amanda LoGuidiceRobert Deans

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