PMID: 6408101Jul 1, 1983Paper

Stimulation and inhibition of secretion in Paramecium: role of divalent cations

The Journal of Cell Biology
D M Gilligan, B H Satir

Abstract

The effects of Ca2+ and Mg2+ on exocytosis in Paramecium tetraurelia cells were examined with light microscopy, freeze fracture (FEM) and transmission electron microscopy (TEM) of thin-sectioned embedded cells. Picric acid-Ca2+-induced secretion in wild type (wt) cells was captured by "quick" fixation with OsO4, and TEM demonstrated membrane fusion occurring before trichocyst matrix (tmx) expansion. Cells stimulated with picric acid in the presence of high extracellular Mg2+ showed very few sites of membrane fusion and no tmx expansion, suggesting that Ca2+ is required for both membrane fusion and tmx expansion. Further information was obtained by comparing secretory responses of wt cells with a temperature-sensitive secretory mutant, nd 9. These cells when grown at the permissive temperature (18 degrees C) possess normal rosettes at the secretory site and secrete in response to picric acid-Ca2+, but when grown at 27 degrees C they lack rosettes and do not secrete (Beisson, J., M. Lefort-Tran, M. Pouphile, M. Rossignol, and B. Satir, 1976, J. Cell Biol., 69:126-143). Quantitation of picric acid-Ca2+-induced secretion revealed that: (a) the number of tmx secreted by wt and nd 9 cells was independent of their cultural growth phas...Continue Reading

References

Feb 3, 1978·Science·B H Satir, S G Oberg
Nov 1, 1972·Journal of Ultrastructure Research·F Wunderlich, V Speth
Nov 1, 1968·British Journal of Pharmacology·W W Douglas
Dec 1, 1981·The Journal of Cell Biology·J J Rauh, D L Nelson
Apr 1, 1983·The Journal of Cell Biology·R S GarofaloB H Satir
Jan 1, 1980·Annals of the New York Academy of Sciences·B H SatirN J Maihle

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Citations

Jan 27, 2005·The Anatomical Record. Part B, the New Anatomist·Robert B Silver, George D Pappas
Dec 1, 1991·Developmental Biology·C J Merkle, D E Chandler
May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amélie LeforestierFrançoise Livolant
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S V Subramanian, B H Satir
Apr 2, 1998·European Journal of Cell Biology·H Zhao, B H Satir
Jun 29, 1990·European Journal of Protistology·B Haacke-BellH Plattner
Jul 1, 1989·The Journal of protozoology·B H Satir
Apr 1, 1988·Journal of Cellular Biochemistry·B H SatirT J Murtaugh
Dec 25, 1984·Journal of Molecular Biology·A AdoutteJ Beisson
Jul 10, 2019·Fish & Shellfish Immunology·Iria FolgueiraJosé Manuel Leiro
Dec 1, 1996·Microbiological Reviews·B Becker, M Melkonian

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