Stimulation by P2X₇ receptors of calcium-dependent production of reactive oxygen species (ROS) in rat submandibular glands

Biochimica Et Biophysica Acta
Unai FontanilsAida Marino

Abstract

Agonists of P2X₇ receptors increase the production of reactive oxygen species (ROS) in immunocytes. In this work we tested this response and its effect on mitochondrial inner membrane potential (Deltapsi(m)) in exocrine glands. The production of ROS by rat submandibular glands was investigated by measuring the oxidation of dichlorodihydrofluorescein (DCFH), a fluorescent probe. The Deltapsi(m) was estimated with tetramethylrhodamine. Activation of P2X₇ receptors by ATP or Bz-ATP increased the production of ROS. This response was not modified by inhibitors of phospholipase A2 or of various kinases. The effect of ATP was calcium-dependent and was blocked by diphenyliodonium, an inhibitor of flavoproteins. It was not affected by rotenone, an inhibitor of the complex I of the mitochondrial electron transfer chain. Scavengers of ROS had no effect on the dissipation of Deltaψ(m) by ATP. We conclude that, in rat submandibular glands, P2X₇ receptors stimulate in a calcium-dependent manner an oxidase generating ROS, suggesting the involvement of the dual oxidase Duox2. The production of ROS does not contribute to the depolarization of mitochondria by purinergic agonists. Purinergic receptors could be regulators of the bactericidal prope...Continue Reading

Citations

Sep 28, 2012·Purinergic Signalling·Bin Wang, Ronald Sluyter
Oct 29, 2013·Antioxidants & Redox Signaling·Xavier De DekenFrançoise Miot
Aug 16, 2013·Antioxidants & Redox Signaling·Stanko S StojilkovicClaudio Coddou
Dec 20, 2014·Purinergic Signalling·Débora A GonzálezMariano A Ostuni
Jun 5, 2013·Médecine sciences : M/S·Stéphanie PochetJean-Paul Dehaye
Oct 22, 2020·International Journal of Molecular Sciences·Seung-Woo KimJa-Kyeong Lee
Jan 6, 2021·Bioorganic & Medicinal Chemistry·Evgeny PislyaginDmitry Aminin

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