PMID: 40151Oct 1, 1979

Stimulation by subsynaptosomal fractions of transmitter efflux from plain synaptic vesicle fraction

Neurochemical Research
M Takeda, R Tanaka


The effects were investigated of purified subsynaptic fractions on the efflux of radioactivity from a plain synaptic vesicle fraction which had incorporated [3H]dopamine. About 50% of the radioactivity incorporated into the plain vesicles (120 microgram protein) was liberated on exposure to purified synaptic membranes (30 microgram protein). The synaptic membrane-dependent efflux appeared to depend on both adenosine triphosphate and divalent cations, especially Ca2+. Of the sub-cellular fractions used, the heavy microsomal fraction showed the same effects as the synaptic membrane fraction. Purified synaptic junctions exhibited the strongest stimulating effects: the efflux was 2 times greater than that observed with synaptic membranes. The stimulating effects of myelin were less than one-seventh of those of synaptic junctional fraction. These observations may indicate that the transmitters are liberated by interaction of vesicle membrane with synaptic membrane in the presence of ATP and divalent cations.


Jan 1, 1976·Cold Spring Harbor Symposia on Quantitative Biology·R RahamimoffH Rahamimoff
Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Sep 1, 1965·Archives of Biochemistry and Biophysics·R Tanaka, K P Strickland
Dec 3, 1971·Biochimica Et Biophysica Acta·C W Cotman, D A Matthews
Oct 1, 1973·Journal of Neurochemistry·W T Norton, S E Poduslo
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M P BlausteinP Needleman
Dec 1, 1972·The Journal of Cell Biology·C W Cotman, D Taylor
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Apr 1, 1970·Life Sciences·A Philippu, W Heyd
Jan 1, 1967·Biochimica Et Biophysica Acta·P CerlettiR Strom
Dec 1, 1963·Biochemical Pharmacology·R J WURTMAN, J AXELROD


Oct 8, 1990·Biochimica Et Biophysica Acta·P D Thomas, G J Brewer

Related Concepts

Cell Membrane Permeability
Edetic Acid, Sodium Salt
Egtazic Acid Sodium Salt
Intracellular Membranes
Synaptic Vesicles

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.