PMID: 40151Oct 1, 1979

Stimulation by subsynaptosomal fractions of transmitter efflux from plain synaptic vesicle fraction

Neurochemical Research
M Takeda, R Tanaka

Abstract

The effects were investigated of purified subsynaptic fractions on the efflux of radioactivity from a plain synaptic vesicle fraction which had incorporated [3H]dopamine. About 50% of the radioactivity incorporated into the plain vesicles (120 microgram protein) was liberated on exposure to purified synaptic membranes (30 microgram protein). The synaptic membrane-dependent efflux appeared to depend on both adenosine triphosphate and divalent cations, especially Ca2+. Of the sub-cellular fractions used, the heavy microsomal fraction showed the same effects as the synaptic membrane fraction. Purified synaptic junctions exhibited the strongest stimulating effects: the efflux was 2 times greater than that observed with synaptic membranes. The stimulating effects of myelin were less than one-seventh of those of synaptic junctional fraction. These observations may indicate that the transmitters are liberated by interaction of vesicle membrane with synaptic membrane in the presence of ATP and divalent cations.

References

Jan 1, 1976·Cold Spring Harbor Symposia on Quantitative Biology·R RahamimoffH Rahamimoff
Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Sep 1, 1965·Archives of Biochemistry and Biophysics·R Tanaka, K P Strickland
Dec 3, 1971·Biochimica Et Biophysica Acta·C W Cotman, D A Matthews
Oct 1, 1973·Journal of Neurochemistry·W T Norton, S E Poduslo
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M P BlausteinP Needleman
Dec 1, 1972·The Journal of Cell Biology·C W Cotman, D Taylor
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Apr 1, 1970·Life Sciences·A Philippu, W Heyd
Jan 1, 1967·Biochimica Et Biophysica Acta·P CerlettiR Strom
Dec 1, 1963·Biochemical Pharmacology·R J WURTMAN, J AXELROD

Citations

Oct 8, 1990·Biochimica Et Biophysica Acta·P D Thomas, G J Brewer

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