Stimulation of 5-HT2A receptors on astrocytes in primary culture opens voltage-independent Ca2+ channels

Neurochemistry International
G B HagbergE Hansson

Abstract

Mechanisms underlying the 5-HT2A receptor induction of intracellular Ca2+ mobilization and Ca2+ influx in type I astroglial cells in primary culture from newborn rat cerebral cortex were evaluated. The 5-HT-evoked Ca(2+)-transients, inhibited by the 5-HT2A antagonists ketanserin or 4-(4-fluorobenzoyl)-1-(4-phenylbutyl) piperidine oxalate, consisted of an initial peak caused by inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ release from internal stores, and a second sustained part which was due to Ca2+ transport over the plasma membrane. The responses were pertussis toxin-insensitive, suppressed by the phospholipase C inhibitor neomycin and were inhibited by the Ca(2+)-ATPase inhibitor thapsigargin. Furthermore, the responses were inhibited by the IP3 receptor antagonist heparin. When the second sustained part of the 5-HT-evoked response was studied, it was concluded that Ca2+ influx was not a result of opening of voltage operated calcium channels of either L, N or T-type. Instead it appeared that Ca2+ entered the cells through specialized voltage independent Ca2+ channels which were dependent of the IP3 production and subsequent Ca2+ release from internal stores. From this, we conclude that 5-HT opens Ca2+ channels in astrocy...Continue Reading

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Citations

Mar 28, 2002·Pharmacology & Therapeutics·J R RaymondM N Garnovskaya
Jun 15, 2007·Teaching and Learning in Medicine·Janice L Hanson, Virginia F Randall
Oct 27, 2015·Frontiers in Pharmacology·Gongliang Zhang, Robert W Stackman
Oct 27, 2004·Pharmacology & Therapeutics·Takafumi NagatomoTadazumi Komiyama
Jan 27, 2018·Nature Communications·Shahriar SheikhbahaeiAlexander V Gourine
Apr 18, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Cecilia Flores, Joseph T Coyle
Oct 3, 2013·The European Journal of Neuroscience·Linda BlockElisabeth Hansson
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elisabeth Hansson, Lars Rönnbäck
Dec 22, 1999·Journal of Neurophysiology·F M Zhou, J J Hablitz
Oct 13, 2021·Translational Psychiatry·Judit Cabana-DomínguezBru Cormand
Nov 25, 2021·Journal of Neurochemistry·Hannah N Saeger, David E Olson

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