Stimulation of acetylcholine output from brain slices caused by the ionophores BrX-537A and A23187

British Journal of Pharmacology
F CasamentiG Pepeu


1 The effect of two ionophores, BrX-537A (Bromolasolacid) and A 23187, on acetylcholine (ACh) output from brain slices was studied. 2 The slices were prepared from rat cerebral cortex, incubated in Krebs solution containing physostigmine and ACh output determined by bioassay. 3 Both ionophores enhanced ACh output. BrX-537A exerted its maximal effect, a six fold increase, at a concentration of 1.8 micron, while A 23187 caused a three fold increase at a concentration of 58 micron. 4 When the slices were incubated in a Ca-free medium, the effect of A 23187 on ACh output was only reduced, BrX-537A was abolished while that of BrX-537A was also active when disodium edetate (EDTA) was added to the the Ca-free medium. 5 The activity of BrX-537A was not affected by the presence of tetrodotoxin in the incubation medium. 6 The stimulation of ACh output elicited by KCl (25 mM) was increased further by hyoscine, but not by BrX-537A. Hyoscine however had no effect when ACh output was stimulated by BrX-537A. 7 The effect of BrX-537A on ACh output was potentiated by the addition of Mg2+ (9.3 mM) to the incubation medium and was reduced in a Mg-free medium. 8 It is concluded that A 23187 stimulates ACh output by transporting extracellular Ca2+ ...Continue Reading


Jul 1, 1976·The Journal of Physiology·H Kita, W Van Der Kloot
Aug 1, 1976·The Journal of Physiology·S G Cull-CandyS Thesleff
Sep 24, 1976·Science·I KaoD L Price
Feb 20, 1975·Nature·D I Devore, W L Nastuk
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D E Cochrane, W W Douglas
Nov 1, 1970·British Journal of Pharmacology·P C Molenaar, R L Polak
Jun 1, 1968·British Journal of Pharmacology and Chemotherapy·M M Bertels-Meeuws, R L Polak
Sep 1, 1967·British Journal of Pharmacology and Chemotherapy·A Bartolini, G Pepeu
Sep 1, 1967·International Journal of Neuropharmacology·M B Bowers
Nov 1, 1966·The Journal of General Physiology·W D Dettbarn, P Rosenberg
May 1, 1964·The Journal of General Physiology·T NARAHASHIW R SCOTT
Feb 16, 1965·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·B KATZ, R MILEDI

Related Concepts

Acetilcolina Cusi
Bacteriocidal Agents
Edetic Acid, Calcium, Sodium Salt
Cavia porcellus

Related Feeds

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.