Stimulation of Alpha-Adrenoceptors Facilitates the Release of Growth Hormone-Releasing Hormone from Rat Hypothalamus in vitro

Journal of Neuroendocrinology
S TsagarakisA Grossman

Abstract

Abstract While extensive evidence suggests that adrenoceptors play an important role in the control of growth hormone in the rat, there are few studies involving the direct measurement of growth hormone-releasing hormone (GHRH). We have therefore developed a radioimmunoassay for rat GHRH, and used it to investigate the modulation of GHRH release by noradrenaline from incubated rat hypothalamus in vitro. The GHRH radioimmunoassay had no significant cross-reactivity with other hypothalamic or GHRH-related peptides, and was sensitive to 4 pg/tube; intra- and interassay coefficients of variation were 6% and 12% respectively. Single incubated rat hypothalami produced a stable and readily measurable output of GHRH in successive 20 min incubations after an initial 60 min preincubation; the release of GHRH was increased in the presence of 56 mM KCI, but did not respond to KCI-depolarization when calcium was excluded from the medium. Stimulated GHRH release was identical to synthetic rat GHRH(1-43) on high-performance liquid chromatography and Sephadex G-75 chromatography. Noradrenaline stimulated GHRH secretion in a dose-dependent manner in the concentration range 10(-10)- 10(-6)M, with a plateau in response at 10(-7)M. Stimulation wit...Continue Reading

Citations

Feb 1, 1976·Clinical Pharmacology and Therapeutics·M R AchongR I Ogilvie
Nov 1, 1985·Endocrinology·S N PerkinsM J Cronin
Jul 1, 1987·British Journal of Clinical Pharmacology·S Al-DamlujiG M Besser
Apr 1, 1983·Naunyn-Schmiedeberg's Archives of Pharmacology·J A HannahJ L Reid

Related Concepts

Calcium [EPC]
Growth Hormone Activity
Calcium
Chromatography
Methoxamine
Idazoxan
Process of Secretion
Adrenergic Receptor
Norepinephrine
Norepinephrine Measurement

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