Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.

BMC Cancer
Ulrich JoimelClaudine Soria

Abstract

Infiltration by macrophages (Mphi) indicates a poor prognosis in breast cancers, in particular by inducing angiogenesis. Our study aimed 1) to investigate the mechanism by which cooperation between Mphi and aggressive breast cancer cells (MDA-MB-231) induces angiogenesis; 2) to examine the effect of tetrathiomolybdate (TM) on this angiogenic activity. Mphi coincubated with MDA-MB-231 were used as a model to mimic the inflammatory microenvironment. Angiogenesis induced by the culture media was tested in the chick chorioallantoic membrane (CAM). Mphi phenotype was evaluated by 1) expression of the M1 marker CD80, and secretion of interleukin 10 (IL-10), an M2 marker; 2) capacity to secrete Tumour Necrosis Factor alpha (TNFalpha) when stimulated by lipopolysaccharide/interferon gamma (LPS/IFNgamma); 3) ability to induce MDA-MB-231 apoptosis. To explore the molecular mechanisms involved, cytokine profiles of conditioned media from MDA-MB-231, Mphi and the coculture were characterised by an antibody cytokine array. All experiments were carried out both in presence and in absence of TM. Incubation of Mphi with MDA-MB-231 induced a pro-angiogenic effect in the CAM. It emerged that the angiogenic activity of the coculture is due to the...Continue Reading

References

Aug 1, 1988·Journal of Leukocyte Biology·E H HelinskiJ L Pauly
May 1, 1995·Journal of Leukocyte Biology·R M StrieterS L Kunkel
Nov 10, 1995·The Journal of Biological Chemistry·R M StrieterD Marriott
Jul 1, 1994·The Journal of Experimental Medicine·N RomaniG Schuler
Jun 15, 1996·The Journal of Clinical Investigation·D A ArenbergR M Strieter
Nov 19, 1997·Journal of Leukocyte Biology·D A ArenbergR M Strieter
Aug 6, 1998·The Journal of Clinical Investigation·D A ArenbergR M Strieter
Nov 5, 1998·The Journal of Clinical Investigation·J BohuslavR J Ulevitch
Jan 11, 2000·International Journal of Cancer. Journal International Du Cancer·H TorisuM Kuwano
Jan 27, 2000·Journal of Leukocyte Biology·H HaghnegahdarA Richmond
May 24, 2000·Cancer Immunology, Immunotherapy : CII·D A ArenbergR M Strieter
Feb 7, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Von Knethen A, B Brüne
Mar 21, 2001·The Journal of Experimental Medicine·E Y LinJ W Pollard
Jun 18, 2002·Clinical & Experimental Metastasis·Claudio BrigatiRoberto Benelli
Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Jun 5, 2003·Expert Opinion on Therapeutic Targets·Mary SproullKevin Camphausen
Oct 25, 2003·Immunobiology·Eva GottfriedMarina Kreutz
Dec 11, 2003·Integrative Cancer Therapies·George J Brewer, Sofia D Merajver
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael P KeaneRobert M Strieter
May 28, 2004·Endocrine-related Cancer·V L GoodmanS D Merajver
May 17, 2005·Current Cancer Drug Targets·George J Brewer
Jun 7, 2005·American Journal of Physiology. Heart and Circulatory Physiology·David F SmithYuqing Huo
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thorsten HagemannFrances R Balkwill
Mar 3, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Robert M StrieterJohn A Belperio
Mar 8, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Antonio SicaPaola Allavena
Jul 25, 2006·The Journal of Clinical Investigation·Yunping LuoRong Xiang
Aug 18, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jose C JuarezFernando Doñate
Aug 23, 2006·Journal of Mammary Gland Biology and Neoplasia·Sarah A Lowndes, Adrian L Harris
Sep 13, 2006·Cancer Metastasis Reviews·Alberto MantovaniAntonio Sica
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fernando O MartinezAlberto Mantovani
Nov 23, 2006·Cancer Research·Elaine Y LinJeffrey W Pollard
Apr 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alana L WelmJ Michael Bishop
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Fernando Oneissi MartinezMassimo Locati
Jan 23, 2008·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·A CarpenterC Erkuran-Yilmaz

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Citations

Nov 8, 2011·Breast Cancer Research and Treatment· HamidullahRituraj Konwar
May 15, 2012·Pathology Oncology Research : POR·Aleksandra JethonMarzena Podhorska-Okolow
Feb 5, 2016·Biochimica Et Biophysica Acta·Tazzio TissotFrédéric Thomas
Feb 6, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Tazzio TissotFrédéric Thomas
Apr 27, 2012·Veterinary and Comparative Oncology·T RaposoF L Queiroga
May 20, 2018·Scientific Reports·Marcio MatsumotoRaquel C R Martinez
Jun 30, 2019·International Journal of Molecular Sciences·Hwan Hee LeeHyosun Cho
Nov 30, 2018·Chemical Reviews·Bernhard EnglingerWalter Berger

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Methods Mentioned

BETA
FCS
Enzyme-Linked Immunosorbent Assay
Antibody Array
X-ray
ELISA

Software Mentioned

Saisam
imageJ

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