Stimulation of arachidonic acid metabolism and generation of thromboxane A2 by leukotrienes B4, C4 and D4 in guinea-pig lung in vitro

British Journal of Pharmacology
P J Piper, M N Samhoun

Abstract

1 Leukotriene C4 (LTC4), LTD4, slow-reacting substance of anaphylaxis (SRS-A) (from guinea-pig lung), bradykinin (Bk) and arachidonic acid (AA) release thromboxane A2 (TxA2) and prostaglandin-like materials from guinea-pig isolated perfused lungs. 2 Release of TxA2 induced by LTC4 and LTD4 is inhibited by a thromboxane synthetase inhibitor, imidazole (2.9 mM). 3 Mepacrine (200 microM), a phospholipase inhibitor, inhibits release of TxA2 and prostaglandin-like materials caused by SRS-A and Bk but not that due to exogenous AA 4 Leukotrienes B4, C4 and D4 are approximately equipotent in inducing dose-related contractions of guinea-pig parenchymal strips (GPPs). 5 Leukotriene-induced contractions of GPPs are greatly inhibited by imidazole (2.9 mM), carboxyheptylimidazole (24 microM) and mepacrine (400 microM). 6 FPL 55712 (1.9 microM), the SRS-A antagonist, blocks contractions of GPPs induced by LTC4 and LTD4 but not those due to LTB4 or Bk. 7 Tachyphylaxis to LTB4 occurs in GPPs but not to LTC4 or LTD4. 8 These results suggest that in guinea-pig lung in vitro, LTB4, LTC4 and LTD4 activate a phospholipase with subsequent generation of cyclo-oxygenase products of which TxA2 plays an important role.

References

Jan 1, 1979·Agents and Actions. Supplements·H R MorrisJ R Tippins
Sep 1, 1977·Prostaglandins·J SvenssenM Hamberg
Mar 1, 1976·European Journal of Pharmacology·M A Wasserman
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H R MorrisJ R Tippins
Feb 12, 1980·Biochemical and Biophysical Research Communications·O RådmarkE J Corey
Dec 1, 1980·Prostaglandins and Medicine·P SiroisG Girard
Jun 1, 1960·British Journal of Pharmacology and Chemotherapy·H O COLLIERP G SHORLEY
Aug 1, 1964·British Journal of Pharmacology and Chemotherapy·P A BERRY, H O COLLIER

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Citations

Mar 1, 1986·Inflammation·D E GriswoldS Mong
Nov 1, 1991·Agents and Actions·S JancarP Sirois
Jan 1, 1989·Agents and Actions·E WikströmS E Dahlén
Aug 7, 1999·Clinical Reviews in Allergy & Immunology·N C Barnes, L J Smith
Aug 7, 1999·Clinical Reviews in Allergy & Immunology·S E Dahlén
Jan 29, 1986·European Journal of Pharmacology·J P SealeJ Shaw
Oct 11, 1988·European Journal of Pharmacology·I Ahnfelt-RønneC Kaergaard-Nielsen
Mar 1, 1983·Prostaglandins·R Hamel, A W Ford-Hutchinson
Jan 1, 1986·Prostaglandins·G B GraybarP J Kadowitz
May 1, 1988·Prostaglandins·J P CristolP Sirois
Jan 1, 1989·Pharmacology & Therapeutics·P J Gardiner
Jan 1, 1984·Prostaglandins, Leukotrienes, and Medicine·M N Samhoun, P J Piper
Dec 1, 1987·Prostaglandins, Leukotrienes, and Medicine·H NagaiM Nakazawa
Jul 1, 1990·Journal of Ethnopharmacology·M E Addy, J F Burka
Oct 31, 1998·European Journal of Pharmacology·E Wikström JonssonS E Dahlén
Apr 17, 1999·Journal of Internal Medicine·H E Claesson, S E Dahlén
Jul 26, 1984·The New England Journal of Medicine·E J Goetzl
Aug 1, 1987·Annals of Surgery·H AnnerH B Hechtman
Dec 1, 1996·Thorax·P E Christie, N C Barnes
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·S E Dahlén
Jul 1, 1987·The American Review of Respiratory Disease·J G GarciaA B Malik
May 1, 1988·The American Review of Respiratory Disease·D P SkonerP Fireman
Jun 1, 1988·The American Review of Respiratory Disease·I S FarrukhG H Gurtner
Dec 24, 2008·Prostaglandins & Other Lipid Mediators·Ewa SelgSven-Erik Dahlén

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