PMID: 2117444Jul 15, 1990Paper

Stimulation of Ca2(+)-independent catecholamine secretion from digitonin-permeabilized bovine adrenal chromaffin cells by guanine nucleotide analogues. Relationship to arachidonate release

The Biochemical Journal
A Morgan, R D Burgoyne

Abstract

The effect of GTP analogues on catecholamine secretion and [3H]arachidonic acid release from digitonin-permeabilized adrenal chromaffin cells was examined. Several GTP analogues stimulated Ca2(+)-independent exocytosis, with the order of efficacy being XTP greater than ITP greater than guanosine 5'-[beta gamma-imido]triphosphate (p[NH]ppG) greater than guanosine 5'-[gamma-thio]triphosphate (GTP[S]). The stimulatory effect of the GTP analogues appeared to be due to activation of a conventional GTP-binding protein, as it was inhibited by guanosine 5'-[beta-thio]diphosphate (GDP[S]). In contrast, Ca2(+)-dependent exocytosis was only partially inhibited by high doses of GDP[S]. GTP did not stimulate Ca2(+)-independent exocytosis, but instead was found to inhibit secretion caused by micromolar Ca2+. Arachidonic acid (100 microM) also stimulated Ca2(+)-independent catecholamine secretion. Determination of the effect of GTP analogues on release of free [3H]arachidonic acid into the medium showed that it was stimulated by GTP[S] but inhibited by GTP, p[NH]ppG, ITP and XTP. The inhibition of [3H]arachidonic acid release by XTP was not prevented by GDP[S]. These results demonstrate that activation of a GTP-binding protein by certain GTP ...Continue Reading

Citations

Jan 1, 1991·Annals of the New York Academy of Sciences·R W HolzM A Bittner
Oct 1, 1992·The European Journal of Neuroscience·Jean-Marie SontagMarie-France Bader
Apr 1, 1999·Annual Review of Physiology·J AveryJ M Edwardson
Mar 9, 1994·Annals of the New York Academy of Sciences·R D BurgoyneD Roth
Mar 29, 2003·Physiological Reviews·Robert D Burgoyne, Alan Morgan
Jul 22, 1991·Biochimica Et Biophysica Acta·R D Burgoyne
Dec 12, 1991·Biochimica Et Biophysica Acta·M Lindau, B D Gomperts

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