Stimulation of calcium uptake by 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) in rabbit platelets: possible involvement of the lipoxygenase pathway

Archives of Biochemistry and Biophysics
T C LeeF Snyder

Abstract

1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) induces an increase of Ca2+ uptake in rabbit platelets. This process depends upon the extracellular concentration of Ca2+ with the maximum stimulation occurring at 1-3 mM; uptake under these conditions is blocked by verapamil, a calcium-entry blocker. Increase of calcium uptake by the bioactive phospholipid was independent of ADP-induced platelet responses and of metabolites of arachidonic acid metabolism formed through the cyclooxygenase pathway. However, mepacrine, p-bromophenacyl bromide, eicosatetraynoic acid, and nordihydroguaiaretic acid significantly or totally inhibited the stimulation of Ca2+ uptake by 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine. When arachidonic acid was given sufficient time to be metabolized to other products by the platelets, stimulation of Ca2+ uptake also occurred. Arachidonic acid and platelet-activating factor did not produce an additive or synergistic effect. Our data suggest that a metabolite(s) generated from arachidonic acid through the lipoxygenase pathway may be the mediator(s) responsible for the action of platelet-activating factor in the induction of increased Ca2+ uptake in rabbit platelets.

References

Oct 29, 1979·Biochemical and Biophysical Research Communications·M L BlankE E Muirhead
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Hamberg, B Samuelsson
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson
Mar 26, 1974·Biochemistry·J J VolwerkG H de Haas
May 1, 1981·The Journal of Clinical Investigation·P H NaccacheE J Goetzl
Oct 30, 1981·Biochemical and Biophysical Research Communications·T C LeeF Snyder
Apr 15, 1982·Archives of Biochemistry and Biophysics·S L HofmannP W Majerus
Feb 15, 1981·Biochemical Pharmacology·B B VargaftigJ Benveniste
Feb 27, 1980·Biochemical and Biophysical Research Communications·M VolpiR I Sha'afi

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Citations

Jan 1, 1986·The Journal of Membrane Biology·M C Sekar, L E Hokin
Sep 1, 1986·Basic Research in Cardiology·J NimpfT Kenner
Jun 1, 1987·Agents and Actions·E R PettipherB Henderson
Oct 15, 1984·European Journal of Pharmacology·P IñarreaM S Crespo
Apr 27, 1988·European Journal of Pharmacology·M A MartinsB B Vargaftig
Apr 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S S EdwinM D Mitchell
May 25, 2010·Photochemistry and Photobiology·Jared B TraversJeffrey B Travers
Feb 18, 1998·The Journal of Pharmacy and Pharmacology·C H LiaoC M Teng
Mar 1, 1988·Journal of Pharmacological Methods·A CalignanoP Persico
Mar 1, 1989·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·A H MohammedR Dziak
Feb 1, 1989·Experimental and Molecular Pathology·A NosedaE J Modest
Jan 1, 1985·Clinical and Experimental Hypertension. Part A, Theory and Practice·Z Zukowska-GrojecG Feuerstein
Sep 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·P MentzH J Mest
Jul 14, 1989·Biochemical and Biophysical Research Communications·M T AlonsoJ García-Sancho

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