Stimulation of Camel Polyclonal Antibody against Human T cell Immunoglobulin and Mucin 3

Iranian Journal of Biotechnology
Vida HomayouniAbbas Rezaei

Abstract

Background: T cell Immunoglobulin, Mucin (TIM)-3, is a type I transmembrane glycoprotein belonging to TIM family. This receptor expresses on T helper type 1 (Th1) cells that binds to galectin-9 (Gal9); inducing an inhibitory signal. As a result, apoptosis of Th1 cells occurs and cytotoxicity of CD8 T cells becomes evident in vitro. Therefore, this immunomodulatory molecule may be used as a novel target for clinical purposes. The production of camel polyclonal antibodies against TIM-3-expressing cell line was the purpose of this study. Objectives: In this study, we aimed to use HEK 293 cells expressing human TIM-3 to obtain camel polyclonal antibody against TIM-3 by immunization. Materials and Methods: A pre-synthesized human TIM-3cDNA was inserted into pcDNA3.1 plasmid and the new construct was transfected in HEK cell. TIM-3 expression was confirmed by qRT-PCR and flow cytometry. A camel (6 months old) was immunized with the lysate prepared from rTIM-3 expressing HEK cells 4 times. The anti-TIM-3 antibody level was evaluated using ELISA method. Results: TIM-3 was successfully cloned in HEK cells with 88% success rate. High level of anti-TIM-3 antibody was detected in the serum of the camel immunized with the recombinant cell ly...Continue Reading

References

Aug 31, 2001·Journal of Biotechnology·S Muyldermans
May 3, 2005·Journal of Pharmacological and Toxicological Methods·Philip Thomas, Trevor G Smart
Sep 9, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christophe MariatXin Xiao Zheng
Feb 20, 2008·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Masako SekiMitsuomi Hirashima
Sep 22, 2009·Molecular Biology Reports·Hai-Feng PanDong-Qing Ye
Jun 12, 2010·Immunological Reviews·Gordon J FreemanRosemarie H DeKruyff
Sep 8, 2010·The Journal of Experimental Medicine·Kaori SakuishiAna C Anderson
Jun 24, 2011·Trends in Immunology·Kaori SakuishiVijay K Kuchroo
Sep 13, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M Y YeungN Najafian
Nov 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shalini SharmaBarry T Rouse
Jan 10, 2012·Current Opinion in Immunology·Ana Carrizosa Anderson
May 16, 2012·American Journal of Clinical Pathology·Xuewei ZhuangChunhong Ma
Sep 4, 2012·Protein Expression and Purification·Zhizhong ChenLihua Hu
Feb 11, 2014·Protein Science : a Publication of the Protein Society·Annamarie C Dalton, William A Barton

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Citations

Aug 28, 2020·Frontiers in Immunology·Lia WalcherUta Kossatz-Boehlert
Aug 15, 2020·Frontiers in Oncology·Emily Y Yang, Khalid Shah
Jan 12, 2021·Frontiers in Oncology·Mazdak Ganjalikhani HakemiAlexandr V Bazhin

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