PMID: 6982738Nov 1, 1982Paper

Stimulation of canine lymphocyte subpopulations separated nonlytically by monoclonal anti-T and polyclonal anti-B cell antibodies

Blut
J C WulffR Storb

Abstract

Canine blood lymphocytes were nonlytically separated on antibody-coated petri dishes into surface immunoglobulin-positive (SIg+) and -negative (SIg-) populations. SIg- cells were further separated into cells reactive or non-reactive with monoclonal antibody DT-2 recognizing canine T lymphocytes. The purity of the three enriched lymphocyte populations exceeded 90% as assessed by immunofluorescence. Mitogen stimulation showed a vigorous response of SIg+ cells to pokeweed mitogen and concanavalin A but only a weak response to phytohemagglutinin. In mixed DT-2- and DT-2+ cells responded to phytohemagglutinin, concanavalin A and pokeweed mitogen, and both populations were good responders in mixed leukocyte culture. Only DT-2- cells were potent stimulators; DT-2+ cells were not. Hence, canine blood T cells can be divided into two subsets, DT-2+ and DT-2-, both of which are responsive to mitogens and alloantigens.

Citations

Sep 1, 1986·Veterinary Immunology and Immunopathology·P J FelsburgS I Rubin
Mar 1, 1988·Veterinary Immunology and Immunopathology·G K OgilvieC W Harris
Jan 1, 1991·Tissue Antigens·K KrumbacherH Grosse-Wilde
Jan 1, 1982·Immunogenetics·H J DeegR Storb
Aug 1, 1989·Clinical Immunology and Immunopathology·P F JezykD F Patterson
May 1, 1985·Tissue Antigens·R F DoverenC J van der Linden
Feb 1, 1986·Tissue Antigens·R F DoverenW A Buurman

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