PMID: 7540755Feb 1, 1995Paper

Stimulation of cultured melanocytes in medium containing a serum substitute: Ultroser-G

Pigment Cell Research
N P SmitS Pavel

Abstract

Melanocyte cultures were established and maintained routinely in Ham's F-10 medium containing 12-O-tetradecanoyl-phorbol-13-acetate (TPA), isobutylmethylxanthine (IBMX), cholera toxin (CT) and fetal calf serum (FCS). Three serum substitutes (Ultroser-G, Nutridoma-Hu and Nutricyte-H) were tested in order to obtain a medium without FCS having a more constant composition. Melanocyte proliferation was examined in long-term culture experiments by in situ cell counts at different periods of time. Only with Ultroser-G (1-2%) was the proliferation of melanocytes maintained without both FCS and CT, whereas the addition of the other two serum substitutes resulted in stabilization of melanocyte densities in the cultures up to 28 days. In the medium containing 1% Ultroser-G and IBMX without TPA minimal or no increases in melanocyte density were found. Addition of basic fibroblast growth factor (bFGF, 1 ng/ml) to the medium without TPA resulted in a partial restimulation of growth in different experiments. In this system with 1% Ultroser-G and 1 ng/ml bFGF, IBMX could also be replaced by other factors (dbcAMP, LTC4 and a purified form of alpha-melanocyte stimulating hormone). The culture medium with 1% Ultroser-G containing TPA and IBMX is ...Continue Reading

References

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Citations

Dec 24, 1997·Clinics in Dermatology·I C Le Poole, P K Das
Oct 9, 1998·The Journal of Investigative Dermatology·E WenczlA A Schothorst
Dec 24, 1997·The Journal of Investigative Dermatology·N P SmitS Pavel
Jan 18, 2008·The Journal of Investigative Dermatology·N P M SmitJ Van Pelt
Mar 8, 2003·Pigment Cell Research·M J HoogduijnA J Thody

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