Stimulation of cyclic AMP production by the Caenorhabditis elegans muscarinic acetylcholine receptor GAR-3 in Chinese hamster ovary cells

Archives of Biochemistry and Biophysics
Yang-Seo ParkNam Jeong Cho

Abstract

Among the three G-protein-linked acetylcholine receptors (GARs) in Caenorhabditis elegans (C. elegans), GAR-3 is structurally and pharmacologically most similar to mammalian muscarinic acetylcholine receptors (mAChRs). Using Chinese hamster ovary (CHO) cells stably expressing GAR-3b, the major alternatively spliced isoform of GAR-3, we observed that carbachol stimulated cyclic AMP (cAMP) production in a dose- and time-dependent manner. The stimulating effect of carbachol was abolished by atropine, a muscarinic antagonist, indicating that the cAMP production is specifically mediated by GAR-3b. When the cells were treated with BAPTA-AM and EGTA, which reduce the cytosolic Ca(2+) level, carbachol-stimulated cAMP accumulation was inhibited by approximately 56%. Inhibition of protein kinase C (PKC) by chronic treatment with phorbol 12-myristate 13-acetate (PMA) or by GF109203X decreased carbachol-stimulated cAMP production by as much as 68%. It thus appears that Ca(2+) and PKC are critically involved in GAR-3b-mediated cAMP formation. We also observed that carbachol-stimulated cAMP production was further enhanced by pertussis toxin (PTX) treatment. This observation indicates that GAR-3b couples to a PTX-sensitive G protein, presumab...Continue Reading

References

Dec 14, 1990·Biochemical and Biophysical Research Communications·M A Buck, C M Fraser
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Aug 23, 2000·Pflügers Archiv : European journal of physiology·K E PembertonS V Jones
Aug 21, 2003·Biochemical and Biophysical Research Communications·Yang-Seo ParkNam Jeong Cho

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Citations

Aug 30, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jason P ChanDerek Sieburth
Apr 23, 2013·Cellular and Molecular Life Sciences : CMLS·Caitlin CollinCornelis J P Grimmelikhuijzen
Sep 20, 2019·Cell Communication and Signaling : CCS·Miron Mikhailowitsch GershkovichSimone Prömel

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