Stimulation of D2 dopamine receptors decreases intracellular calcium levels in rat anterior pituitary cells but not striatal synaptosomes: a flow cytometric study using indo-1.

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M E Wolf, G Kapatos

Abstract

An important question is whether all D2 dopamine (DA) receptors employ the same signal transduction mechanisms. Anterior pituitary cells and striatal synaptosomes, which possess pharmacologically similar D2 DA receptors, were compared with respect to the effect of D2 DA receptor stimulation on free intracellular Ca2+ levels [( Ca2+]i). Flow cytometry, in combination with either the fluorescent calcium indicator indo-1 or fluorescent voltage-sensitive dyes, was used to measure [Ca2+]i and to detect changes in membrane potential. In subpopulations of anterior pituitary cells, increases in [Ca2+]i were produced by elevated K+, veratridine, thyrotropin-releasing hormone, and BAY K 8644. These increases were blocked by nifedipine, suggesting the involvement of L-type voltage-sensitive calcium channels (VSCC's). In 10-15% of the cells, D2 agonists decreased resting [Ca2+]i, reversed stimulus-induced increases in [Ca2+]i, and caused a hyperpolarization. In striatal synaptosomes, elevated K+ and veratridine also increased [Ca2+]i. However, the K+-induced increase was eliminated if choline was substituted for Na+ in the medium, suggesting that Ca2+ entry in response to sustained K+ depolarization resulted from reversal of Na+/Ca2+ excha...Continue Reading

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Nov 4, 2008·Clinical Neuropharmacology·Fabio BlandiniMarco Cosentino
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Sep 14, 2011·Journal of Neuroscience Methods·Danielle Guez-BarberBruce T Hope
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