PMID: 6407527Feb 7, 1983Paper

Stimulation of deacylation in Madin-Darby canine kidney cells. 12-O-tetradecanoyl-phorbol-13-acetate stimulates rapid phospholipid deacylation

Biochimica Et Biophysica Acta
G A BeaudryM Waite

Abstract

The tumor-promoting phorbol diester, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), stimulates Madin-Darby canine (MDCK) cells to deacylate cellular phospholipid and to produce prostaglandins. We have used this system to characterize the kinetics of deacylation of [3H]arachidonate and the further metabolism of arachidonate by the cyclooxygenase system. Stimulation of the appearance of [3H]arachidonic acid in extracellular fluids was found to be maximal 2 h after treatment with TPA and its subsequent removal. The production of prostaglandins then followed for up to 24 h. Phospholipase activity was not inhibited by indomethacin over the range of 0.01-100 micrograms/ml. In contrast, prostaglandin synthesis was inhibited at 1 microgram/ml indomethacin. Further, there was a significant stimulation of deacylation within 15 min in the presence of TPA that increased to nearly 30% of the total radioactivity within 1 h. Likewise, stimulation of prostaglandin production was detected within 15 min, but, unlike the deacylation process, did not increase significantly during TPA treatment. The source of arachidonic acid in the early stimulation period was found to be primarily phosphatidylethanolamine, but phosphatidylcholine and phosphatidylin...Continue Reading

References

Dec 18, 1979·Biochimica Et Biophysica Acta·R L Jesse, R C Franson
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L KaplanP Elsbach
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R L BellP W Majerus
Feb 1, 1976·Biochemical Pharmacology·R J Flower, G J Blackwell
Sep 30, 1980·Biochimica Et Biophysica Acta·H van den Bosch
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D R RobinsonM L Stephenson
Jan 29, 1980·Biochemical and Biophysical Research Communications·S D Schimmel, T Hallam
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Feb 17, 1984·European Journal of Pharmacology·S Heisler
Mar 1, 1990·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·G S SchusterB B Singh
Jul 30, 1999·Biochimica Et Biophysica Acta·L W DanielS Ghosh
Jun 25, 1984·Biochimica Et Biophysica Acta·S L Pelech, D E Vance
Mar 1, 1991·Kidney International·J V Bonventre, R Nemenoff

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