Stimulation of development in vitro by platelet-activating factor receptor ligands released by mouse preimplantation embryos

Journal of Reproduction and Fertility
N R StoddartT P Fleming

Abstract

Previous reports have demonstrated that culture of mouse preimplantation embryos at high density stimulates their rate of development. The molecular basis of this phenomenon was investigated. Culture of embryos from the four-cell stage at high density in normal medium, or at low density either in embryo-conditioned medium or medium containing platelet-activating factor (PAF), significantly advanced the timing of compaction, initiation of cavitation and/or completion of zona hatching, and also increased the number of cells in blastocysts. In contrast, Lyso-PAF, an inactive metabolite of PAF, and Enantio-PAF, an enantiomer of PAF, did not have a stimulatory effect at low embryo density, but did not inhibit the stimulation of development at high embryo density. The stimulatory effect of culture at high density was inhibited in the presence of either CV-3988 or SDZ 64-412, two structurally distinct competitive PAF-receptor antagonists, while the development rate at low density was not affected. We conclude that an embryo-derived factor related to PAF is secreted by blastomeres during in vitro culture and acts in a receptor-mediated manner to stimulate the rate of development.

Citations

Dec 15, 2012·Journal of Assisted Reproduction and Genetics·Tao TaoRodney Ouellette
Nov 19, 2005·The Journal of Reproduction and Development·Tatsuo FujitaKazuho Shiga
Feb 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Nadia Gopichandran, Henry J Leese
Oct 15, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Jane C FenelonMarilyn B Renfree
Dec 23, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Michael HoelkerDawit Tesfaye
Mar 2, 2016·Differentiation; Research in Biological Diversity·Hyun Sook KimYong-Pil Cheon
Jun 21, 2005·Developmental Biology·Paula Jane StokesHenry J Leese
Aug 30, 2016·Reproductive Biomedicine Online·Rebecca L Kelley, David K Gardner
Feb 28, 2003·Biology of Reproduction·David P LuChristopher O'Neill
Jan 5, 2002·Molecular Reproduction and Development·Marziel KamjooSusan J Kimber
Mar 26, 2005·Human Reproduction Update·Chris O'Neill
Oct 14, 2017·Clinical and Experimental Reproductive Medicine·Rebecca L Kelley, David K Gardner
Feb 15, 2002·Human Fertility : Journal of the British Fertility Society·Daniel R. Brison

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