Stimulation of fibroblast proliferation by lactate-mediated oxidants

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Silvia WagnerHorst D Becker

Abstract

Lactate accumulation is a characteristic of wounds in which glycolysis, occurring both aerobically and anaerobically, contributes to its production. Cell proliferation is a critical component of healing wounds. Recently it has been shown that lactate can chelate iron and thus promotes production of hydroxyl radicals. We report here that exogenous lactate increases intracellular oxidants and that the oxidants promote cell growth in cultured dermal fibroblasts in a dose-dependent manner. The production of lactate-mediated oxidant requires iron and hydrogen peroxide and with increasing iron concentration oxidant production is raised as well. However, we found cell proliferation is retarded by 15 mM lactate in the presence of a high iron concentration (7.25 microM). The antioxidants catalase and mannitol abolish the inhibitory effect of high lactate. We conclude from these results that increased proliferation of cultured human fibroblasts by exogenous lactate is mediated by oxidant production.

References

Jul 27, 1992·FEBS Letters·B Halliwell, J M Gutteridge
Feb 1, 1990·The Biochemical Journal·G A MurrellL Bromley
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A NovogrodskyK H Stenzel
Apr 5, 1983·Biochimica Et Biophysica Acta·N D McKayN Rooke-Allen
Apr 1, 1983·Journal of Cellular Physiology·F J Darfler, P A Insel
Jun 9, 1995·The Journal of Biological Chemistry·B González-Flecha, B Demple
Feb 1, 1995·Mutation Research·J RemacleG Rao
Sep 25, 1997·Archives of Surgery·D B AllenT K Hunt
Jun 13, 1998·The Journal of Biological Chemistry·J DuranteauP T Schumacker
Oct 9, 1998·The Journal of Biological Chemistry·A K BouzierM Merle
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·L B Gladden
Apr 12, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M ChoM Z Hussain
Apr 21, 2001·Molecular and Cellular Biology·T N SeagrovesR S Johnson
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·R S ArnoldJ D Lambeth
Jun 21, 2001·The Biochemical Journal·K OrinoF M Torti
Jan 5, 2002·Physiological Reviews·Wulf Dröge
Jun 18, 2002·The Journal of Biological Chemistry·Chandan K SenSashwati Roy
Dec 11, 2002·The Journal of Biological Chemistry·Botond BánfiKarl-Heinz Krause
Feb 24, 1956·Science·O WARBURG
Oct 7, 1964·Annals of the New York Academy of Sciences·H KEBERLE

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Citations

May 25, 2005·Histochemistry and Cell Biology·Martin OberringerAndreas Seekamp
Apr 1, 2008·The Journal of Biological Chemistry·Rayudu GopalakrishnaThomas H McNeill
Jun 15, 2007·Antioxidants & Redox Signaling·Thomas K HuntChandan K Sen
Mar 23, 2010·Applied and Environmental Microbiology·Jose M Bruno-BárcenaHosni M Hassan
Jul 11, 2006·Cancer Science·Michael I KoukourakisEfthimios Sivridis
Apr 15, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Amrita Roy, Soumen Bera
Jun 22, 2016·Clinical Oral Investigations·Ioana BaldeaMarioara Moldovan
Jul 13, 2017·Antioxidants & Redox Signaling·Britta Kunkemoeller, Themis R Kyriakides
Aug 29, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Michael I KoukourakisAlexandra Giatromanolaki
Jan 14, 2020·American Journal of Physiology. Renal Physiology·Yan ShenJunwei Yang
Feb 27, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ilaria GiustiVincenza Dolo
Feb 11, 2021·The Journal of Physiology·George A BrooksAshley P Tovar
Mar 3, 2020·Redox Biology·George A Brooks
Mar 16, 2019·Biochemical and Biophysical Research Communications·Mingyu KangDo-Yeon Kim
Nov 5, 2021·Frontiers in Physiology·Heather L CaslinJohn J Ryan

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