Stimulation of gelatinase B and tissue inhibitors of metalloproteinase (TIMP) production in co-culture of human osteosarcoma cells and human fibroblasts: gelatinase B production was stimulated via up-regulation of fibroblast growth factor (FGF) receptor

International Journal of Cancer. Journal International Du Cancer
T KurogiM Koono

Abstract

Co-cultures of human osteosarcoma Takase (OST) cells with various human fibroblasts derived from surgical specimens stimulated production of gelatinase B (92-kDa type-IV collagenase, MMP-9), tissue inhibitors of metalloproteinase (TIMP)-1, and TIMP-2 when compared to cultures of individual cells. The maximum stimulation of gelatinase-B production occurred at a cellular ratio of 1:1. Conditioned media from several fibroblast cultures stimulated OST cells to produce gelatinase B, TIMP-1 and TIMP-2, but not vice versa. Among various recombinant growth factors or cytokines, tumor necrosis factor (TNF)-alpha stimulated gelatinase-B production in cultures of OST cells alone, while recombinant basic fibroblast growth factor (bFGF) stimulated gelatinase-B production in co-cultures of OST cells with skin fibroblasts but not in individual cultures of each cell type. In the co-cultures, gelatinase-B production was inhibited by anti-bFGF monoclonal antibody (MAb), but not by anti TNF-alpha MAb. This co-culture-specific stimulation of gelatinase-B production by bFGF was associated with increased expression of the FGF receptor in the co-culture.

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Citations

Jan 30, 2014·Cancers·Antonietta Rosella Farina, Andrew Reay Mackay
Apr 4, 2002·Biological Chemistry·Olivier De Wever, Marc Mareel
Jan 7, 2003·Hematology/oncology Clinics of North America·W Thomas PurcellManuel Hidalgo
Jun 11, 2002·Biochemical and Biophysical Research Communications·Jian-Feng LiuDominique Ledoux
Aug 30, 2000·International Journal of Cancer. Journal International Du Cancer·T SameshimaS Wakisaka
Feb 7, 2001·Journal of the National Cancer Institute·M Hidalgo, S G Eckhardt

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