Stimulation of glucose transport by insulin and norepinephrine in isolated rat brown adipocytes.

The American Journal of Physiology
A Marette, L J Bukowiecki

Abstract

The effects of insulin and norepinephrine on glucose transport, glucose uptake, and cell respiration were investigated in isolated rat brown adipocytes. Glucose transport and uptake were determined using [U-14C]-D-glucose and 2-deoxy-[1,2-3H]-D-glucose, respectively. Brown adipocyte respiration was measured polarographically. Dose-response experiments revealed that insulin stimulated D-glucose transport and 2-deoxyglucose uptake between 10(-11) and 10(-7) M with a maximal four- to sixfold stimulation. In the absence of insulin, norepinephrine concentrations ranging from 10(-7) to 10(-7) M also enhanced glucose transport and uptake with a maximal two- to fourfold stimulation. Experiments with alpha- and beta-adrenergic agonists and antagonists showed that the effect of norepinephrine was predominantly mediated via beta-adrenergic pathways. Dibutyryl cyclic AMP and 3-isobutyl-1-methylxanthine also increased glucose transport, suggesting that the effects of norepinephrine are cyclic AMP dependent. Moreover, norepinephrine (10(-8) M) enhanced insulin sensitivity for glucose transport [half-maximum velocity constant (1/2 V max)] but failed to potentiate insulin responsiveness (Vmax). On the other hand, insulin (10(-9) M) had no effe...Continue Reading

References

Mar 1, 1986·Diabetes·A L VallerandL J Bukowiecki
Oct 1, 1987·The Biochemical Journal·R Greco-PerottoB Jeanrenaud
May 1, 1986·Canadian Journal of Physiology and Pharmacology·S W Ma, D O Foster
Sep 1, 1985·The American Journal of Physiology·H Wallberg-Henriksson, J O Holloszy
May 22, 1984·Biochimica Et Biophysica Acta·J GliemannJ A Foley

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Citations

Apr 30, 1998·Physiological Reviews·M Florez-Duquet, R B McDonald
Apr 8, 2006·Physiological Reviews·J Enrique Silva
Dec 25, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Sunhee KimChun K Kim
Dec 25, 2009·Endocrinology·Steven T Russell, Michael J Tisdale
Sep 18, 2015·Frontiers in Endocrinology·Wei Zhang, Sheng Bi
May 26, 2016·European Journal of Endocrinology·Moe Thuzar, Ken K Y Ho
Jun 9, 2017·Hormone Molecular Biology and Clinical Investigation·Peter AldissMichael E Symonds
Oct 12, 2010·International Journal of Obesity : Journal of the International Association for the Study of Obesity·B Cannon, J Nedergaard
Nov 12, 2014·The Journal of Cell Biology·Jessica M OlsenTore Bengtsson
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·Stefan KraussBradford B Lowell
May 3, 2007·American Journal of Physiology. Endocrinology and Metabolism·Jan NedergaardBarbara Cannon
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Sep 22, 2010·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S T Russell, M J Tisdale
Feb 1, 1995·British Journal of Pharmacology·Y L Liu, M J Stock
Oct 8, 2020·Cell Reports·Caryn CarsonHeather A Lawson
Mar 14, 2001·European Journal of Pharmacology·M J García-BarradoJ Moratinos
Apr 1, 1995·Physiology & Behavior·B C BockJ R Aprille
May 10, 2021·Molecular Metabolism·Alice ÅslundTore Bengtsson
Nov 1, 1992·Molecular and Cellular Endocrinology·C Olichon-BertheY Le Marchand-Brustel
Mar 1, 1992·Diabetes Research and Clinical Practice·M IgarashiH Sasaki
Jun 30, 1992·Biochemical and Biophysical Research Communications·H NikamiM Saito

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